US 12,235,202 B2
Flow cytometer performance evaluation method and standard particle suspension
Keiji Nakagawa, Tokyo (JP)
Assigned to ThinkCyte K.K., Tokyo (JP)
Filed by ThinkCyte K.K., Tokyo (JP)
Filed on Jun. 23, 2022, as Appl. No. 17/847,478.
Application 17/847,478 is a continuation of application No. PCT/JP2020/048503, filed on Dec. 24, 2020.
Claims priority of application No. 2019-238089 (JP), filed on Dec. 27, 2019.
Prior Publication US 2022/0317020 A1, Oct. 6, 2022
Int. Cl. G01N 15/14 (2024.01); G01N 15/10 (2024.01); G01N 15/149 (2024.01)
CPC G01N 15/1468 (2013.01) [G01N 15/1012 (2013.01); G01N 15/1459 (2013.01); G01N 2015/1014 (2024.01); G01N 15/149 (2024.01)] 21 Claims
OG exemplary drawing
 
1. A method of evaluating performance of a flow cytometer configured to use two or more types of calibration particles having different morphologies from each other in combination, comprising:
a first discrimination step of discriminating the two or more types of calibration particles from each other based on a first optical characteristic by the flow cytometer, wherein the flow cytometer is an evaluation target;
a second discrimination step of discriminating the two or more types of calibration particles from each other based on a second optical characteristic which is discriminable at a spatial resolution lower than a spatial resolution at which the first optical characteristic is discriminated; and
an evaluation step of evaluating one or both of a particle discrimination performance and a resolution of the flow cytometer based on a first discrimination result assessed in the first discrimination step and a second discrimination result assessed in the second discrimination step, wherein the two or more types of calibration particles further have third optical characteristics which are substantially same as each other.