US 12,235,201 B2
Methods for continuous measurement of baseline noise in a flow cytometer and systems for same
Peter Mage, Goleta, CA (US); Lingjie Wei, Milpitas, CA (US); Peter Johnson, Santa Cruz, CA (US); David A. Roberts, San Jose, CA (US); and Keegan Owsley, Campbell, CA (US)
Assigned to BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed by Becton, Dickinson and Company, Franklin Lakes, CA (US)
Filed on Aug. 16, 2021, as Appl. No. 17/403,105.
Claims priority of provisional application 63/081,660, filed on Sep. 22, 2020.
Prior Publication US 2022/0091017 A1, Mar. 24, 2022
Int. Cl. G01N 15/10 (2024.01); G01N 15/14 (2024.01); G01N 15/1429 (2024.01); G01N 21/64 (2006.01); G01N 15/149 (2024.01)
CPC G01N 15/1012 (2013.01) [G01N 15/1429 (2013.01); G01N 15/1459 (2013.01); G01N 2015/1402 (2013.01); G01N 15/149 (2024.01); G01N 21/6456 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for determining baseline noise of a photodetector in a particle analyzer, the method comprising:
irradiating a sample comprising particles in a flow stream;
detecting light with a photodetector from a particle-free component of the irradiated flow stream;
generating data signals from the detected light;
calculating a moving average mean squared error of the generated data signals by measuring a squared difference between the generated data signals and baseline data signals; and
determining the baseline noise of the photodetector based on the calculated mean squared error of the generated data signals over a predetermined period of time.