US 12,235,196 B2
Method for pretreatment of biological sample
Toshiya Matsubara, Columbia, MD (US)
Assigned to SHIMADZU CORPORATION, Kyoto (JP)
Appl. No. 17/264,887
Filed by SHIMADZU CORPORATION, Kyoto (JP)
PCT Filed Sep. 18, 2018, PCT No. PCT/JP2018/034434
§ 371(c)(1), (2) Date Feb. 1, 2021,
PCT Pub. No. WO2020/059013, PCT Pub. Date Mar. 26, 2020.
Prior Publication US 2021/0302286 A1, Sep. 30, 2021
Int. Cl. G01N 1/38 (2006.01); G01N 33/68 (2006.01)
CPC G01N 1/38 (2013.01) [G01N 33/6848 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A method for pretreatment of a biological sample in order to measure a protein contained in the biological sample by liquid chromatography/mass spectrometry, the method comprising the steps of:
adding an acetic acid aqueous solution to the biological sample to prepare a pretreatment sample containing an acetic acid at a concentration ranging from 20 to 50 weight percent, both inclusive; and thereafter
adding acetic acid aqueous solution to the pretreatment sample, wherein the pretreatment sample has undergone a pretreatment, to prepare an analysis sample which contains acetic acid at a concentration ranging from 20 to 50 weight percent, both inclusive.