US 12,234,573 B2
Library of antigen-binding molecules including modified antibody variable region
Tomoyuki Igawa, Shizuoka (JP); Shojiro Kadono, Kanagawa (JP); Naoka Hironiwa, Shizuoka (JP); and Mika Sakurai, Shizuoka (JP)
Assigned to Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
Filed by CHUGAI SEIYAKU KABUSHIKI KAISHA, Tokyo (JP)
Filed on Oct. 21, 2021, as Appl. No. 17/506,733.
Application 17/506,733 is a division of application No. 15/525,603, granted, now 11,154,615, previously published as PCT/JP2015/081693, filed on Nov. 11, 2015.
Claims priority of application No. PCT/JP2014/079785 (WO), filed on Nov. 11, 2014; and application No. 2015-097884 (JP), filed on May 13, 2015.
Prior Publication US 2022/0040297 A1, Feb. 10, 2022
Int. Cl. C40B 40/10 (2006.01); A61K 39/395 (2006.01); C07K 16/28 (2006.01); C07K 16/42 (2006.01); C12N 15/09 (2006.01); C40B 30/04 (2006.01); C40B 40/08 (2006.01)
CPC C40B 40/10 (2013.01) [A61K 39/395 (2013.01); C07K 16/28 (2013.01); C07K 16/2809 (2013.01); C07K 16/2848 (2013.01); C07K 16/2866 (2013.01); C07K 16/2875 (2013.01); C07K 16/2896 (2013.01); C07K 16/42 (2013.01); C12N 15/09 (2013.01); C40B 30/04 (2013.01); C40B 40/08 (2013.01); C07K 2317/31 (2013.01); C07K 2317/35 (2013.01); C07K 2317/55 (2013.01); C07K 2317/92 (2013.01)] 12 Claims
OG exemplary drawing
 
1. An antigen-binding polypeptide molecule comprising an antibody variable region that binds to a first, a second, and a third antigen,
wherein the first, the second, and the third antigens are three different antigens;
wherein each of the three different antigens binds to a site on the antibody variable region that is not identical to the site on the antibody variable region bound by either of the other two antigens, though any of the sites may overlap with any other;
wherein the antibody variable region cannot bind to more than two of the three different antigens at the same time;
wherein the first antigen is CD3;
wherein the antibody variable region comprises (i) a VH comprising SEQ ID NO: 13, 52, 59, 94, 96, or 97 with one or more amino acid changes, or (ii) a VL comprising SEQ ID NO: 53 with one or more amino acid changes, or both (i) and (ii);
wherein the one or more amino acid changes comprise one or more substitutions or insertions or both;
wherein the one or more amino acid changes create binding sites for the second and third antigens; and
wherein the one or more amino acid changes are at a position or positions selected from VH positions 31 to 35, 50 to 65, 71 to 74, and 95 to 102, and VL positions 24 to 34, 50 to 56, and 89 to 97, wherein all position numbers are according to the Kabat numbering system.