US 12,234,511 B2
Methods of analyte detection
Bryan P. Staker, San Ramon, CA (US); Niandong Liu, San Ramon, CA (US); Michael David McLaughlin, San Jose, CA (US); and Bart Lee Staker, Poulsbo, WA (US)
Assigned to Pacific Biosciences of California, Inc., Menlo Park, CA (US)
Filed by Pacific Biosciences of California, Inc., Menlo Park, CA (US)
Filed on Dec. 21, 2021, as Appl. No. 17/557,715.
Application 17/557,715 is a continuation of application No. 17/061,023, filed on Oct. 1, 2020, granted, now 11,248,266.
Application 17/061,023 is a continuation of application No. 16/572,535, filed on Sep. 16, 2019, granted, now 10,829,816, issued on Nov. 10, 2020.
Application 16/572,535 is a continuation in part of application No. 16/458,977, filed on Jul. 1, 2019, granted, now 11,060,140, issued on Jul. 13, 2021.
Application 16/458,977 is a continuation of application No. 15/925,656, filed on Mar. 19, 2018, granted, now 10,378,053, issued on Aug. 13, 2019.
Application 16/572,535 is a continuation in part of application No. 14/443,655, abandoned, previously published as PCT/US2013/070797, filed on Nov. 19, 2013.
Claims priority of provisional application 62/473,163, filed on Mar. 17, 2017.
Claims priority of provisional application 61/869,020, filed on Aug. 22, 2013.
Claims priority of provisional application 61/728,067, filed on Nov. 19, 2012.
Prior Publication US 2024/0052413 A1, Feb. 15, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. G16B 30/00 (2019.01); C12Q 1/6874 (2018.01); G06T 7/73 (2017.01); G06V 20/69 (2022.01); G16B 25/30 (2019.01)
CPC C12Q 1/6874 (2013.01) [G06T 7/73 (2017.01); G06V 20/69 (2022.01); G16B 25/30 (2019.02); G16B 30/00 (2019.02); G06F 2218/18 (2023.01); G06T 2207/20056 (2013.01); G06T 2207/30072 (2013.01); G06V 20/695 (2022.01)] 20 Claims
OG exemplary drawing
 
1. A method of identifying an analyte, comprising:
(a) providing at least one analyte of a plurality of analytes, wherein said at least one analyte of said plurality of analytes is immobilized on a substrate;
(b) performing a plurality of cycles of binding one or more probes to said at least one analyte of said plurality of analytes, wherein said plurality of cycles of probe binding generates a sequence of optical signals;
(c) detecting said sequence of optical signals with an imaging system, wherein said imaging system has about 2 pixels per μm2 of said substrate; and
(d) processing said sequence of optical signals with an expected sequence of optical signals corresponding to said at least one analyte of said plurality of analytes to generate a decoded sequence of optical signals, wherein said decoded sequence of optical signals identifies said at least one analyte of said plurality of analytes with an error rate less than 5% over at least 20 cycles of said plurality of cycles.