US 12,234,507 B2
Compositions and methods for targeted masking of autofluorescence
Hong Chen, Dublin, CA (US); Joshua Delaney, Northcote (AU); Yi Luo, Dublin, CA (US); and Meiliana Tjandra, Dublin, CA (US)
Assigned to 10X GENOMICS, INC., Pleasanton, CA (US)
Filed by 10x Genomics, Inc., Pleasanton, CA (US)
Filed on Aug. 7, 2023, as Appl. No. 18/366,621.
Application 18/366,621 is a continuation of application No. PCT/US2023/017152, filed on Mar. 31, 2023.
Claims priority of provisional application 63/326,698, filed on Apr. 1, 2022.
Prior Publication US 2024/0043914 A1, Feb. 8, 2024
Int. Cl. C12Q 1/6841 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6876 (2018.01); G01N 1/30 (2006.01); G01N 21/64 (2006.01)
CPC C12Q 1/6841 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6876 (2013.01); G01N 1/30 (2013.01); G01N 21/64 (2013.01); G01N 2021/6432 (2013.01); G01N 2021/6439 (2013.01)] 30 Claims
 
1. A method, comprising:
(a) contacting a biological sample with a quencher comprising a quencher dye and a targeting moiety, wherein the targeting moiety covalently binds with a biological moiety endogenous to the biological sample, thereby targeting the quencher to the biological moiety;
(b) contacting the biological sample with a detectable probe that binds to a molecule of interest in the biological sample; and
(c) detecting a signal associated with the detectable probe bound to the molecule of interest in the biological sample,
thereby detecting the molecule of interest in the biological sample.