US 12,234,504 B1
High throughput screening assay to identify DNA topoisomerase inhibitors
Fenfei Leng, Palmetto Bay, FL (US); and Matthew Dias, Miami, FL (US)
Assigned to The Florida International University Board of Trustees, Miami, FL (US)
Filed by Fenfei Leng, Palmetto Bay, FL (US); and Matthew Dias, Miami, FL (US)
Filed on Oct. 1, 2024, as Appl. No. 18/903,582.
Claims priority of provisional application 63/544,373, filed on Oct. 16, 2023.
Int. Cl. C12Q 1/682 (2018.01); C12Q 1/37 (2006.01); C12Q 1/44 (2006.01); C12Q 1/533 (2006.01); C12Q 1/6832 (2018.01)
CPC C12Q 1/682 (2013.01) [C12Q 1/37 (2013.01); C12Q 1/44 (2013.01); C12Q 1/533 (2013.01); C12Q 1/6832 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for identifying an inhibitor targeting a DNA topoisomerase in a sample, the method comprising adding a circular double-stranded plasmid to the sample; adding the DNA topoisomerase; adding a detergent and a proteinase; adding an exonuclease; adding a DNA-staining dye; and determining the presence or absence of the inhibitor based on fluorescence in the sample.