| CPC C12Q 1/6809 (2013.01) [C12Q 1/6837 (2013.01); G01N 15/1433 (2024.01)] | 20 Claims |
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1. A method of selectively assembling oligonucleotides on a microelectrode array coated with a plurality of anchor sequences, the method comprising:
introducing multiple copies of a first oligonucleotide complex having two sticky ends and encoding a first arbitrary value into a solution in a reaction chamber containing the microelectrode array;
incubating the multiple copies of the first oligonucleotide complex with the microelectrode array so that the multiple copies of the first oligonucleotide complex hybridize to the anchor sequences;
inhibiting hybridization of a first subset of the multiple copies of the first oligonucleotide complex through changing local pH by activating a first subset of electrodes on the microelectrode array such that double-stranded oligonucleotides de-hybridize at the first subset of electrodes but not at other locations on the surface of the microelectrode array; and
closing nicks between ones of the multiple copies of the first oligonucleotide complex that remain hybridized to the anchor sequences thereby forming assembled oligonucleotides.
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