US 12,234,493 B2
Compositions for saccharification of cellulosic material
Brett McBrayer, Sacramento, CA (US); Elena Vlasenko, Davis, CA (US); and Tarana Shaghasi, Guerneville, CA (US)
Assigned to Novozymes, Inc., Davis, CA (US)
Filed by Novozymes, Inc., Davis, CA (US)
Filed on Jun. 13, 2023, as Appl. No. 18/333,776.
Application 16/840,012 is a division of application No. 16/115,930, filed on Aug. 29, 2018, granted, now 10,648,009, issued on May 12, 2020.
Application 16/115,930 is a division of application No. 15/422,245, filed on Feb. 1, 2017, granted, now 10,072,280, issued on Sep. 11, 2018.
Application 15/422,245 is a division of application No. 14/885,555, filed on Oct. 16, 2015, granted, now 9,587,262, issued on Mar. 7, 2017.
Application 14/885,555 is a division of application No. 14/052,360, filed on Oct. 11, 2013, granted, now 9,175,277, issued on Nov. 3, 2015.
Application 14/052,360 is a division of application No. 12/940,952, filed on Nov. 5, 2010, granted, now 8,580,536, issued on Nov. 12, 2013.
Application 18/333,776 is a continuation of application No. 17/372,667, filed on Jul. 12, 2021, granted, now 11,713,476.
Application 17/372,667 is a continuation of application No. 16/840,012, filed on Apr. 3, 2020, granted, now 11,091,785, issued on Aug. 17, 2021.
Claims priority of provisional application 61/259,014, filed on Nov. 6, 2009.
Prior Publication US 2024/0018560 A1, Jan. 18, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/14 (2006.01); C12N 1/22 (2006.01); C12N 9/24 (2006.01); C12N 9/42 (2006.01); C12P 19/02 (2006.01); C12P 19/12 (2006.01); C12P 21/02 (2006.01)
CPC C12P 19/14 (2013.01) [C12N 1/22 (2013.01); C12N 9/2402 (2013.01); C12N 9/2437 (2013.01); C12N 9/2445 (2013.01); C12N 9/248 (2013.01); C12P 19/02 (2013.01); C12P 19/12 (2013.01); C12P 21/02 (2013.01); C12Y 302/01 (2013.01); C12Y 302/01004 (2013.01); C12Y 302/01008 (2013.01); C12Y 302/01021 (2013.01); C12Y 302/01091 (2013.01); C12P 2203/00 (2013.01); C12Y 302/01037 (2013.01); Y02P 20/52 (2015.11)] 55 Claims
 
1. An isolated recombinant host cell encoding an enzyme composition comprising:
(I) a polypeptide having cellobiohydrolase I activity selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 158; (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 157, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO: 157, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 5; and (d) a polypeptide comprising the mature polypeptide of SEQ ID NO: 6;
(II) a polypeptide having beta-glucosidase activity; and
(III) a polypeptide having endoglucanase I activity is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 62; (b) a polypeptide encoded by a polynucleotide that hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 61, (ii) the genomic DNA sequence of the mature polypeptide coding sequence of SEQ ID NO: 61, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 61; and (d) a polypeptide comprising the mature polypeptide of SEQ ID NO: 62.