US 12,234,492 B2
Microorganism for fermentative production of 2-phenylethanol from gaseous substrates
Fungmin Liew, Skokie, IL (US); Michael Koepke, Skokie, IL (US); Shilpa Nagaraju, Skokie, IL (US); and Audrey Harris, Skokie, IL (US)
Assigned to LanzaTech, Inc., Skokie, IL (US)
Filed by LanzaTech, Inc., Skokie, IL (US)
Filed on Jan. 15, 2021, as Appl. No. 17/150,900.
Claims priority of provisional application 62/991,428, filed on Mar. 18, 2020.
Prior Publication US 2021/0292732 A1, Sep. 23, 2021
Int. Cl. C12N 9/88 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/06 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/90 (2006.01); C12P 7/22 (2006.01)
CPC C12N 9/88 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/0016 (2013.01); C12N 9/1085 (2013.01); C12N 9/1092 (2013.01); C12N 9/1205 (2013.01); C12N 9/1294 (2013.01); C12N 9/90 (2013.01); C12P 7/22 (2013.01); C12Y 101/01025 (2013.01); C12Y 102/07005 (2013.01); C12Y 205/01019 (2013.01); C12Y 207/01071 (2013.01); C12Y 207/09001 (2013.01); C12Y 401/01043 (2013.01); C12Y 402/0101 (2013.01); C12Y 402/01051 (2013.01); C12Y 402/03004 (2013.01); C12Y 402/03005 (2013.01); C12Y 504/99005 (2013.01)] 13 Claims
 
1. A C1-fixing microorganism that produces 2-phenylethanol, wherein the microorganism comprises:
(a) a heterologous enzyme that converts phenylpyruvate to phenylacetaldehyde, wherein the enzyme is a decarboxylase; and
(b) a heterologous enzyme that converts phenylacetaldehyde to 2-phenylethanol, wherein the enzyme is a phenylacetaldehyde; wherein the heterologous enzyme in (a) is encoded by a nucleic acid with at least 90% identity to SEQ ID NO: 1, 2, or 4; and the heterologous enzyme in (b) is encoded by a nucleic acid with at least 90% identity to SEQ ID NO: 3, 5, 6, 7, 8, or 9; and wherein the microorganism is a Wood-Ljungdahl microorganism.