	US 12,234,485 B2
	Resin film formed by scaffold material for cell culture and container for cell culture
Yuuhei Arai, Osaka (JP); Ryoma Ishii, Tokyo (JP); Hiroki Iguchi, Osaka (JP); and Satoshi Haneda, Osaka (JP)
Assigned to SEKISUI CHEMICAL CO., LTD., Osaka (JP)
Appl. No. 17/608,587
Filed by SEKISUI CHEMICAL CO., LTD., Osaka (JP)
PCT Filed May 27, 2020, PCT No. PCT/JP2020/020891 § 371(c)(1), (2) Date Nov. 3, 2021, PCT Pub. No. WO2020/241675, PCT Pub. Date Dec. 3, 2020.
Claims priority of application No. 2019-099569 (JP), filed on May 28, 2019.
Prior Publication US 2022/0228127 A1, Jul. 21, 2022
Int. Cl. C12N 5/074 (2010.01)

CPC C12N 5/0696 (2013.01) [C12N 2513/00 (2013.01); C12N 2533/30 (2013.01)]

11 Claims

1. A method of culturing a cell, comprising a step of culturing a cell on a resin film formed of a scaffold material containing a synthetic resin,

the resin film having a compressive modulus of 5.5 GPa or more and 15 GPa or less at a frequency of 1 Hz as measured using a nanoindenter device in ion-exchanged water in accordance with ISO14577-1 after being immersed in the ion-exchanged water at 37° C. for 24 hours, and

the resin film having a contact angle in water of less than 145° and 100° or more in ion-exchanged water at 16° C.