	US 12,234,484 B2
	Culture medium for pluripotent stem cells
Hagen Wieland, Heidelberg (DE)
Assigned to PROMOCELL GMBH, Heidelberg (DE)
Appl. No. 16/096,806
Filed by PROMOCELL GMBH, Heidelberg (DE)
PCT Filed May 18, 2018, PCT No. PCT/EP2018/063036 § 371(c)(1), (2) Date Oct. 26, 2018, PCT Pub. No. WO2018/219684, PCT Pub. Date Dec. 6, 2018.
Claims priority of application No. 17173816 (EP), filed on May 31, 2017.
Prior Publication US 2020/0332263 A1, Oct. 22, 2020
Int. Cl. C12N 5/074 (2010.01)

CPC C12N 5/0696 (2013.01) [C12N 2500/05 (2013.01); C12N 2500/30 (2013.01); C12N 2500/36 (2013.01); C12N 2500/38 (2013.01); C12N 2500/98 (2013.01); C12N 2501/15 (2013.01); C12N 2501/16 (2013.01); C12N 2501/999 (2013.01); C12N 2533/90 (2013.01)]

18 Claims

1. A chemically defined medium for eukaryotic cell culture, comprising water, at least one carbon source, one or more vitamins, one or more salts, one or more fatty acids, one or more buffer components, selenium, at least one substance of the group of Functional Inhibitors of Acid Sphingomyelinase (FIASMAs) and at least one polypeptide of the TGF-β superfamily with the ability to inhibit stem cell differentiation,

wherein the chemically defined medium is free of FGF-2,

wherein the chemically defined medium is completely devoid of human- or animal-derived substances,

wherein the ratio between said FIASMA and said at least one polypeptide of the TGF-β superfamily is from 400:1 to 2000:1,

wherein the concentration of the at least one FIASMA is in the range of 0.1 mg/L to 10 mg/L, and

wherein the concentration of the at least one polypeptide of the TGF-β superfamily is in the range of 0.2 μg/L to 20 μg/L.