US 12,234,482 B2
Culture medium for expanding and culturing human liver progenitor cells and application thereof
Yinxiong Li, Guangzhou (CN); Tingcai Pan, Guangzhou (CN); Yan Chen, Guangzhou (CN); Yuanqi Zhuang, Guangzhou (CN); and Fan Yang, Guangzhou (CN)
Assigned to GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCES, Guangzhou (CN)
Appl. No. 17/310,193
Filed by Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou (CN)
PCT Filed Dec. 19, 2019, PCT No. PCT/CN2019/126578
§ 371(c)(1), (2) Date Jul. 26, 2021,
PCT Pub. No. WO2020/151418, PCT Pub. Date Jul. 30, 2020.
Claims priority of application No. 201910073515.7 (CN), filed on Jan. 25, 2019.
Prior Publication US 2022/0186181 A1, Jun. 16, 2022
Int. Cl. C12N 5/071 (2010.01)
CPC C12N 5/067 (2013.01) [C12N 2500/25 (2013.01); C12N 2501/11 (2013.01); C12N 2501/12 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/41 (2013.01); C12N 2501/73 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A culture medium for expanding and culturing human hepatoblasts, comprising:
(a) a liquid basal culture medium,
(b) an insulin-transferrin-sodium selenite mixture,
(c) a cytokine,
(d) a glycogen synthase kinase 3β inhibitor,
(e) a Hedgehog signaling pathway activator, and
(f) a transforming growth factor β receptor inhibitor.