US 12,234,479 B2
Bifunctional molecule and use thereof
Shuai Chen, Shanghai (CN); Huaxing Zhu, Shanghai (CN); and Yuanping Liao, Shanghai (CN)
Assigned to CYTOCARES (SHANGHAI) INC., Shanghai (CN)
Appl. No. 16/474,554
Filed by CYTOCARES (SHANGHAI) INC., Shanghai (CN)
PCT Filed Aug. 9, 2017, PCT No. PCT/CN2017/096592
§ 371(c)(1), (2) Date Nov. 12, 2019,
PCT Pub. No. WO2018/120842, PCT Pub. Date Jul. 5, 2018.
Claims priority of application No. 201611256643.8 (CN), filed on Dec. 30, 2016; application No. 201611258667.7 (CN), filed on Dec. 30, 2016; application No. 201611260781.3 (CN), filed on Dec. 30, 2016; and application No. 201611260818.2 (CN), filed on Dec. 30, 2016.
Prior Publication US 2023/0242876 A1, Aug. 3, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 16/28 (2006.01); C12N 5/0783 (2010.01); C12N 15/85 (2006.01)
CPC C12N 5/0636 (2013.01) [C07K 16/2803 (2013.01); C07K 16/2809 (2013.01); C07K 16/2818 (2013.01); C07K 16/2827 (2013.01); C07K 16/2875 (2013.01); C07K 16/2878 (2013.01); C12N 15/85 (2013.01); C07K 2317/31 (2013.01); C07K 2317/622 (2013.01); C07K 2317/74 (2013.01); C12N 2501/51 (2013.01); C12N 2501/515 (2013.01); C12N 2501/599 (2013.01); C12N 2800/107 (2013.01)] 10 Claims
 
1. A bifunctional molecule comprising a first function domain capable of binding to and activating a CD3 molecule on the surface of T cell, and a second function domain capable of binding to and activating a T cell positive costimulatory molecule;
wherein the first function domain is an anti-CD3 scFv, the second function domain is a scFv against a T cell positive costimulatory molecule or a ligand extracellular domain of a T cell positive costimulatory molecule;
wherein the first function domain and the second function domain are connected by a linker fragment;
wherein the linker fragment is a hinge domain fragment of immunoglobulin IgD, and the amino acid sequence of the hinge domain fragment of immunoglobulin IgD is as shown in SEQ ID NO. 19.