	US 12,234,467 B2
	Diplospory gene
Peter Johannes Van Dijk, Wageningen (NL); Diana Rigola, Wageningen (NL); Marinus Willem Prins, Wageningen (NL); and Adrianus Johannes Van Tunen, Wageningen (NL)
Assigned to Keygene N.V., Wageningen (NL)
Filed by KEYGENE N.V., Wageningen (NL)
Filed on Dec. 22, 2021, as Appl. No. 17/559,924.
Application 17/559,924 is a continuation of application No. 15/757,020, granted, now 11,236,346, previously published as PCT/NL2016/050617, filed on Sep. 5, 2016.
Claims priority of application No. 2015398 (NL), filed on Sep. 4, 2015.
Prior Publication US 2022/0186238 A1, Jun. 16, 2022
Int. Cl. C12N 15/82 (2006.01); C12N 15/113 (2010.01); C12N 15/62 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6895 (2018.01)

CPC C12N 15/8201 (2013.01) [C12N 15/113 (2013.01); C12N 15/625 (2013.01); C12N 15/8202 (2013.01); C12N 15/8223 (2013.01); C12N 15/8287 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6895 (2013.01); C12Q 2525/207 (2013.01); C12Q 2600/13 (2013.01)]

21 Claims

1. A polynucleotide comprising a chimeric gene that comprises:

(a) (i) the nucleic acid sequence of SEQ ID NO: 1 or 2 or a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO: 1 or 2, (ii) a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO: 3 or an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 3, or (iii) a fragment of (i) or (ii) that has at least 90% sequence identity to SEQ ID NO: 4, 5, 6 or 11 or encodes an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 7 or 12;

operably linked to (b) a promoter sequence that is not naturally associated with (a), and wherein the polynucleotide, an expression product thereof, and/or a protein encoded thereby, is capable of providing diplospory function to a plant or plant cell.