US 12,234,465 B2
Methods and microorganisms for the fermentation of methane to multi-carbon compounds
Jeffrey David Orth, Germantown, MD (US); Louis A. Clark, Germantown, MD (US); Lily Yuin Chao, Germantown, MD (US); Na My Trinh, Germantown, MD (US); Christopher Cheyney Farwell, Germantown, MD (US); Xinhua Zhao, Germantown, MD (US); Matthias Helmut Schmalisch, Germantown, MD (US); Grayson Thomas Wawrzyn, Germantown, MD (US); Xuezhi Li, Germantown, MD (US); Mark Anton Held, Germantown, MD (US); Kevin Lee Dietzel, Germantown, MD (US); and James Kealey, Germantown, MD (US)
Assigned to PRECIGEN, INC., Germantown, MD (US)
Filed by PRECIGEN, INC., Germantown, MD (US)
Filed on Jul. 11, 2022, as Appl. No. 17/811,776.
Application 17/811,776 is a continuation of application No. 16/604,425, granted, now 11,421,235, previously published as PCT/US2018/029688, filed on Apr. 27, 2018.
Claims priority of provisional application 62/512,315, filed on May 30, 2017.
Claims priority of provisional application 62/491,683, filed on Apr. 28, 2017.
Prior Publication US 2023/0051667 A1, Feb. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/20 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/88 (2006.01); C12N 15/52 (2006.01); C12N 15/70 (2006.01); C12P 7/00 (2006.01); C12P 7/16 (2006.01); C12P 7/24 (2006.01)
CPC C12N 15/52 (2013.01) [C12N 9/0006 (2013.01); C12N 9/1022 (2013.01); C12N 9/1205 (2013.01); C12N 9/88 (2013.01); C12N 15/70 (2013.01); C12P 7/16 (2013.01); C12P 7/24 (2013.01); C12Y 101/01001 (2013.01); C12Y 101/01086 (2013.01); C12Y 202/01006 (2013.01); C12Y 401/01072 (2013.01); C12Y 402/01009 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A genetically modified microorganism capable of converting a C1 carbon to a multicarbon product, the microorganism comprising: (a) a gene encoding an acetolactate synthase comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 2; (b) a gene encoding a ketol-acid reductoisomerase comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 4; (c) a gene encoding a dihydroxy-acid dehydratase comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 8; and (d) a gene encoding a 2-keto acid decarboxylase comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 12; wherein at least one of the gene(s) is under the control of a rare earth metal switch.