	US 12,234,464 B2
	Biosynthesis of mogrosides
Jeffrey Ian Boucher, Allston, MA (US); Nicholas Flores, Cambridge, MA (US); Jaide Jensen, Cambridge, MA (US); Dayal Saran, Melrose, MA (US); and Jue Wang, Seattle, WA (US)
Assigned to Ginkgo Bioworks, Inc., Boston, MA (US)
Appl. No. 17/292,266
Filed by Ginkgo Bioworks, Inc., Boston, MA (US)
PCT Filed Nov. 9, 2019, PCT No. PCT/US2019/060652 § 371(c)(1), (2) Date May 7, 2021, PCT Pub. No. WO2020/097588, PCT Pub. Date May 14, 2020.
Claims priority of provisional application 62/758,474, filed on Nov. 9, 2018.
Prior Publication US 2021/0403921 A1, Dec. 30, 2021
Int. Cl. C12N 15/52 (2006.01); C12N 9/90 (2006.01); C12N 15/70 (2006.01); C12N 15/81 (2006.01); C12P 19/60 (2006.01)

CPC C12N 15/52 (2013.01) [C12N 9/90 (2013.01); C12N 15/70 (2013.01); C12N 15/81 (2013.01); C12P 19/60 (2013.01); C12Y 504/99033 (2013.01)]

19 Claims

1. A host cell that comprises a heterologous polynucleotide encoding a cucurbitadienol synthase (CDS) enzyme, wherein the heterologous polynucleotide sequence is at least 90% identical to SEQ ID NO: 3 and/or the amino acid sequence of the CDS encoded by the heterologous polynucleotide is at least 90% identical to SEQ ID NO: 43, and wherein the host cell produces 11-hydroxycucurbitadienol, 24-25 epoxy-cucurbitadienol or cucurbitadienol, and wherein the host cell is not a plant cell.