| CPC C12N 15/1136 (2013.01) [A61K 9/1611 (2013.01); A61P 17/02 (2018.01); C07K 14/003 (2013.01); C12N 2310/14 (2013.01); C12N 2310/3181 (2013.01); C12N 2310/351 (2013.01)] | 10 Claims |
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1. A composition comprising: a nucleic acid molecule comprising at least one selected from the group consisting of:
(i) siRNA comprised of a sense RNA having the sequence of SEQ ID NO: 1 and an antisense RNA having the sequence of SEQ ID NO: 2; (ii) dsRNA comprised of a strand having a sequence of SEQ ID NO: 3 and another strand complementary thereto; (iii) dsRNA comprised of a strand having a sequence of SEQ ID NO: 57 and another strand complementary thereto; (iv) dsRNA comprised of a strand having a sequence of SEQ ID NO: 60 and another strand complementary thereto; and
a porous silica particle,
wherein the nucleic acid molecule is loaded on a surface or inside the pores of the porous silica particle; and
the porous silica particle is characterized in that an average pore diameter ranges from 5 to 100 nm, and t, at which an absorbance ratio in the following Equation 1 becomes 1/2, is 24 or more:
At/Ao [Equation 1]
wherein Ao is absorbance of the porous silica particle measured by putting 5 ml of suspension containing 1 mg/ml of porous silica particle into a cylindrical permeable membrane having pores with a pore diameter of 50 kDa:
15 ml of the same solvent as the suspension comes into contact with an outside of the permeable membrane, and the inside/outside of the permeable membrane are horizontally stirred at 60 rpm and at 37° C.;
pH of the suspension is 7.4; and
At indicates absorbance of the porous silica particle measured after lapse of “t” hours since Ao was measured.
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