US 12,234,452 B2
Scaffolded nucleic acid polymer particles and methods of making and using
Wolfgang Hinz, Carlsbad, CA (US); John Leamon, Stonington, CT (US); David Light, Branford, CT (US); and Jonathan M. Rothberg, Guilford, CT (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Apr. 6, 2020, as Appl. No. 16/841,546.
Application 15/846,195 is a division of application No. 14/987,552, filed on Jan. 4, 2016, abandoned.
Application 16/841,546 is a continuation of application No. 16/121,615, filed on Sep. 4, 2018, granted, now 10,612,017.
Application 16/121,615 is a continuation of application No. 15/846,195, filed on Dec. 18, 2017, abandoned.
Application 14/987,552 is a continuation of application No. 14/044,712, filed on Oct. 2, 2013, granted, now 9,249,461, issued on Feb. 2, 2016.
Application 14/044,712 is a continuation of application No. 12/785,685, filed on May 24, 2010, granted, now 8,574,835, issued on Nov. 5, 2013.
Application 12/785,685 is a continuation in part of application No. 12/475,311, filed on May 29, 2009, abandoned.
Application 12/785,685 is a continuation in part of application No. 12/474,897, filed on May 29, 2009, abandoned.
Claims priority of provisional application 61/297,203, filed on Jan. 21, 2010.
Claims priority of provisional application 61/291,788, filed on Dec. 31, 2009.
Claims priority of provisional application 61/263,734, filed on Nov. 23, 2009.
Claims priority of provisional application 61/242,369, filed on Sep. 14, 2009.
Prior Publication US 2020/0239877 A1, Jul. 30, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01); B01J 19/00 (2006.01); C12Q 1/6874 (2018.01); C40B 40/08 (2006.01); C40B 50/06 (2006.01)
CPC C12N 15/1093 (2013.01) [B01J 19/0046 (2013.01); C12Q 1/6874 (2013.01); C40B 40/08 (2013.01); C40B 50/06 (2013.01); B01J 2219/00466 (2013.01); B01J 2219/005 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00722 (2013.01); Y10T 428/2982 (2015.01)] 11 Claims
 
1. A method of sequencing a template nucleic acid comprising:
(a) disposing a particle in a reaction confinement region associated with an electronic-based sensor, wherein the particle includes a non-nucleosidic polymer network and a plurality of template nucleic acids attached to the non-nucleosidic polymer network, the particle having a coefficient of variance of volume of not greater than 15%, wherein the non-nucleosidic polymer network is a polyacrylamide gel, the polymer being permeable to proteins having a size in the range of 50 kilodaltons to 200 kilodaltons;
(b) performing a polymerase extension reaction on a primer annealed to one or more of the plurality of template nucleic acids to incorporate a nucleotide into the primer; and
(c) detecting a signal output in the electronic-based sensor indicative of the incorporation of the nucleotide into the primer.