	US 12,234,446 B2
	Methods for purification of messenger RNA
Michael Heartlein, Lexington, MA (US); Frank DeRosa, Lexington, MA (US); Anusha Dias, Lexington, MA (US); and Shrirang Karve, Lexington, MA (US)
Assigned to TRANSLATE BIO, INC., Waltham, MA (US)
Filed by Translate Bio, Inc., Lexington, MA (US)
Filed on Nov. 24, 2020, as Appl. No. 17/103,439.
Application 17/103,439 is a continuation of application No. 15/936,289, filed on Mar. 26, 2018, granted, now 10,876,104.
Application 15/936,289 is a continuation of application No. 14/775,915, granted, now 9,957,499, issued on May 1, 2018, previously published as PCT/US2014/028441, filed on Mar. 14, 2014.
Claims priority of provisional application 61/784,996, filed on Mar. 14, 2013.
Prior Publication US 2021/0324368 A1, Oct. 21, 2021
Int. Cl. C12N 15/10 (2006.01); C07H 21/02 (2006.01)

CPC C12N 15/10 (2013.01) [C07H 21/02 (2013.01); C12N 15/1017 (2013.01)]

15 Claims

1. A method of purifying messenger RNA (mRNA), comprising:

(a) providing an impure preparation comprising in vitro synthesized mRNA and prematurely aborted RNA sequences;

(b) subjecting the impure preparation comprising in vitro synthesized mRNA to a denaturing condition; and