US 12,234,445 B2
Glycogen-null methanotrophs and uses thereof
Renee M. Saville, Mountain View, CA (US); Joshua A. Silverman, Los Altos Hills, CA (US); Vincent Tang, Menlo Park, CA (US); Eric G. Luning, Sunnyvale, CA (US); Paloma Rueda, Menlo Park, CA (US); Megan Hsi, Menlo Park, CA (US); and Yelena Stegantseva, Menlo Park, CA (US)
Assigned to Calysta, Inc., Menlo Park, CA (US)
Appl. No. 17/293,832
Filed by Calysta, Inc., Menlo Park, CA (US)
PCT Filed Dec. 26, 2019, PCT No. PCT/US2019/068614
§ 371(c)(1), (2) Date May 13, 2021,
PCT Pub. No. WO2020/139974, PCT Pub. Date Jul. 2, 2020.
Claims priority of provisional application 62/785,668, filed on Dec. 27, 2018.
Prior Publication US 2022/0010268 A1, Jan. 13, 2022
Int. Cl. C12N 1/32 (2006.01); C12N 1/30 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/90 (2006.01); C12P 13/04 (2006.01); C12P 21/00 (2006.01)
CPC C12N 1/32 (2013.01) [C12N 1/30 (2013.01); C12N 9/1051 (2013.01); C12N 9/1241 (2013.01); C12N 9/90 (2013.01); C12P 13/04 (2013.01); C12P 21/00 (2013.01); C12Y 204/01011 (2013.01); C12Y 207/07027 (2013.01); C12Y 504/02002 (2013.01); C12N 2500/05 (2013.01); C12N 2500/30 (2013.01)] 33 Claims
 
1. A modified Methylococcus capsulatus, comprising:
(i) a chromosomal knock-out of a glgA2 isoform of a glycogen synthase gene, or
(ii) a chromosomal knock-out of an ADP-glucose pyrophosphorylase gene and a chromosomal knock-out of a glgA2 isoform of a glycogen synthase gene, wherein the modified Methylococcus capsulatus cultured under conditions comprising a non-limiting amount of a C1 substrate produces:
at least about 30% less glycogen as compared to the parent Methylococcus capsulatus cultured under the same conditions; and/or
at least about 5% more crude protein as compared to the parent Methylococcus capsulatus cultured under the same conditions.