US 12,234,443 B2
Cell recovery apparatus, cell recovery method, cell separation system, and cell separation method
Yushi Sato, Tokyo (JP); Masahiro Okanojo, Tokyo (JP); Shangwu Chen, Tokyo (JP); Katsuhiko Nakashima, Tokyo (JP); Ryosuke Takahashi, Tokyo (JP); and Yasuhiko Tada, Tokyo (JP)
Assigned to Resonac Corporation, Tokyo (JP)
Appl. No. 17/418,936
Filed by RESONAC CORPORATION, Tokyo (JP)
PCT Filed Dec. 15, 2020, PCT No. PCT/JP2020/046697
§ 371(c)(1), (2) Date Jun. 28, 2021,
PCT Pub. No. WO2021/181784, PCT Pub. Date Sep. 16, 2021.
Claims priority of application No. 2020-042322 (JP), filed on Mar. 11, 2020.
Prior Publication US 2022/0306980 A1, Sep. 29, 2022
Int. Cl. C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01); C12M 3/06 (2006.01)
CPC C12M 47/02 (2013.01) [C12M 3/06 (2013.01); C12M 25/16 (2013.01); C12M 33/14 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A cell separation system at least comprising:
a first container having a first filter for separating a first removal target from a suspension comprising cells and a removal target including at least the first removal target and a second removal target;
a cell separation apparatus;
a first flow path connecting the first container and the cell separation apparatus; and
a first pump interposed in the first flow path for transferring the suspension from the first container to the cell separation apparatus by decompression suction,
a second container disposed on the upstream side of the first container;
a second flow path connecting the second container and the first container;
a second pump for transferring the suspension from the second container to the first container via the second flow path; and
a third flow path connecting the second pump and the second container;
wherein the first container, the first flow path, the cell separation apparatus, the first pump, the second container and the second flow path are connected in a closed system, p1 wherein the cells are supported on carriers in the suspension;
wherein the cells are detached from the carriers in the second container;
wherein the cells and the carriers are separated by the first filter, and
wherein the cell separation apparatus comprises an outer cylinder, and an inner cylinder accommodated inside the outer cylinder having a side wall on which a large number of pores are formed, and wherein the cell separation apparatus is configured to separate the second removal target and the cells in a storage region between an inner wall of the outer cylinder and the side wall of the inner cylinder.