US 12,234,439 B2
Filter systems for separating microcarriers from cell culture solutions
Jeremy K. Larsen, Providence, UT (US); Jon Q. Coleman, Pleasant View, UT (US); and David S. Rowley, Nibley, UT (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US); and HyClone Laboratories, Inc., Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Jun. 16, 2023, as Appl. No. 18/336,553.
Application 16/389,688 is a division of application No. 15/193,962, filed on Jun. 27, 2016, granted, now 10,301,585, issued on May 28, 2019.
Application 18/336,553 is a continuation of application No. 17/186,553, filed on Feb. 26, 2021, granted, now 11,840,684.
Application 17/186,553 is a continuation of application No. 16/389,688, filed on Apr. 19, 2019, granted, now 10,934,514, issued on Mar. 2, 2021.
Application 15/193,962 is a continuation of application No. 13/449,101, filed on Apr. 17, 2012, granted, now 9,376,655, issued on Jun. 28, 2016.
Claims priority of provisional application 61/540,967, filed on Sep. 29, 2011.
Prior Publication US 2023/0323268 A1, Oct. 12, 2023
Int. Cl. B01D 29/27 (2006.01); B01D 29/13 (2006.01); B01D 35/02 (2006.01); B01D 35/027 (2006.01); B01D 61/14 (2006.01); B01D 63/06 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/26 (2006.01)
CPC C12M 29/04 (2013.01) [B01D 29/13 (2013.01); B01D 29/27 (2013.01); B01D 35/027 (2013.01); B01D 61/147 (2013.01); B01D 63/06 (2013.01); C12M 23/14 (2013.01); C12M 23/26 (2013.01); C12M 25/16 (2013.01); C12M 33/14 (2013.01); C12M 47/02 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A filter assembly for separating microcarriers from a fluid medium, the filter assembly comprising:
a container with a top-end wall, a bottom-end wall, and a side-end wall bounding a compartment adapted to hold a fluid;
an inlet port through which fluid flows into the compartment,
a single outlet port through which fluid flows out of the compartment; and
a filter disposed within the compartment and fluidically separating the inlet and the single outlet, the filter comprising a porous sheet that divides the compartment into an inlet chamber that is fluidly coupled with the inlet port and an outlet chamber that is fluidly coupled with the single outlet port, the filter allowing the fluid medium to pass therethrough to the single outlet port and preventing microcarriers disposed in the fluid medium from passing therethrough;
wherein the filter is spaced away from the side-end wall and is configured to expand upon accumulation of the microcarriers within the filter; and
wherein the filter comprises one of a rigid portion, a semi-rigid portion, or a rigid mounting ring, so as to be suspended from the top-end wall, and such that the filter expands downwardly and outwardly upon the accumulation of the microcarriers within the filter.
 
9. A cell culturing system comprising the filter assembly as recited in claim 1, further comprising a bioreactor fluidly coupled with the inlet port.