US 12,234,438 B2
Biomanufacturing system, method, and 3D bioprinting hardware in a reduced gravity environment
John C. Vellinger, Floyds Knobs, IN (US); Eugene Boland, Louisville, KY (US); Michael A. Kurk, Georgetown, IN (US); Krystal Milliner, Georgetown, IN (US); Nester Samuel Logan, Greenville, IN (US); and Carlos Chang, Greenville, IN (US)
Assigned to REDWIRE SPACE TECHNOLOGIES, INC., Jacksonville, FL (US)
Filed by Techshot, Inc., Greenville, IN (US)
Filed on Jul. 10, 2020, as Appl. No. 16/926,039.
Application 16/926,039 is a continuation of application No. 16/517,106, filed on Jul. 19, 2019, granted, now 11,788,042.
Application 16/517,106 is a continuation in part of application No. 15/225,547, filed on Aug. 1, 2016, granted, now 10,655,096, issued on May 19, 2020.
Claims priority of provisional application 62/199,793, filed on Jul. 31, 2015.
Prior Publication US 2020/0347333 A1, Nov. 5, 2020
Int. Cl. C12M 3/00 (2006.01); B29C 64/364 (2017.01); B33Y 10/00 (2015.01); B33Y 30/00 (2015.01); B33Y 40/00 (2020.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01); C12M 1/42 (2006.01); B29C 64/10 (2017.01); C12N 1/04 (2006.01)
CPC C12M 21/08 (2013.01) [B29C 64/364 (2017.08); B33Y 30/00 (2014.12); B33Y 40/00 (2014.12); C12M 23/40 (2013.01); C12M 29/10 (2013.01); C12M 33/00 (2013.01); C12M 35/02 (2013.01); B29C 64/10 (2017.08); B33Y 10/00 (2014.12); C12M 35/04 (2013.01); C12N 1/04 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method of culturing a printed tissue in a bioreactor in a reduced gravity environment comprising the steps of: providing a reduced gravity environment; providing one or more bioreactors in said reduced gravity environment; printing one or more tissues into said one or more bioreactors; providing one or more perfusions to said one or more printed tissues; providing one or more electrical stimulations to said one or more printed tissues; and providing one or more mechanical stimulations to said one or more printed tissues.