	US 12,234,274 B2
	Use of the CD2 signaling domain in second-generation chimeric antigen receptors
Carl H. June, Merion Station, PA (US); John Scholler, Narberth, PA (US); and Avery D. Posey, Jr., Philadelphia, PA (US)
Assigned to The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Filed by The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Filed on Aug. 5, 2021, as Appl. No. 17/444,519.
Application 16/460,221 is a division of application No. 15/669,562, filed on Aug. 4, 2017, granted, now 10,501,519, issued on Dec. 10, 2019.
Application 15/669,562 is a division of application No. 14/375,999, granted, now 9,783,591, issued on Oct. 10, 2017, previously published as PCT/US2013/027361, filed on Feb. 22, 2013.
Application 17/444,519 is a continuation of application No. 16/460,221, filed on Jul. 2, 2019, granted, now 11,597,754.
Claims priority of provisional application 61/601,907, filed on Feb. 22, 2012.
Prior Publication US 2021/0371495 A1, Dec. 2, 2021
Int. Cl. C07K 14/705 (2006.01); A61K 39/00 (2006.01); C07K 14/725 (2006.01); C07K 16/28 (2006.01); C07K 16/30 (2006.01); C12N 15/85 (2006.01)

CPC C07K 14/7051 (2013.01) [A61K 39/4611 (2023.05); A61K 39/4631 (2023.05); A61K 39/464412 (2023.05); A61K 39/464468 (2023.05); C07K 14/705 (2013.01); C07K 14/70507 (2013.01); C07K 16/2803 (2013.01); C07K 16/30 (2013.01); C12N 15/85 (2013.01); A61K 2239/21 (2023.05); A61K 2239/48 (2023.05); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01)]

23 Claims

1. An artificial nucleic acid sequence encoding a chimeric antigen receptor (CAR), wherein the artificial nucleic acid sequence comprises an in virto transcribed RNA and a 5′ cap and/or an internal ribosome entry site (IRES) sequence, and wherein the CAR comprises:

(a) an antigen binding domain having affinity for prostate specific membrane antigen (PSMA),

(b) a transmembrane domain,

(d) a CD3 zeta signaling domain.