| CPC B01L 3/502738 (2013.01) [C12M 35/08 (2013.01); C12M 47/04 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0622 (2013.01)] | 18 Claims |
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1. A method of physicochemically treating a single cell using an automatic microfluidic device comprising a microfluidic chip and an optical fiber sensing module, the microfluidic chip comprising a fluidic layer, a pneumatic layer, an elastic layer sandwiched between the fluidic layer and the pneumatic layer, and a cell retrieving hole, the fluidic layer comprising a cell treating region and a cell retrieving channel, the cell retrieving hole connected with the cell retrieving channel, and the pneumatic layer comprising a pneumatic micro-valve group configured to control an opening and a closing of each channel in the fluidic layer, the method comprising:
sucking a single cell into the microfluidic chip by applying a negative pressure;
capturing the single cell in the cell treating region;
detecting a cell sensing signal with the optical fiber sensing module as a result of the single cell being captured in the cell treating region;
as a result of the optical fiber sensing module detecting the cell sensing signal when the single cell is captured in the cell treating region, physicochemically treating the single cell sequentially with a first reagent, then with a second reagent, and then with a third reagent, each reagent in different proportions, by controlling the pneumatic micro-valve group; and
backwashing the single cell into the cell retrieving channel with the third reagent used to physicochemically treat the cell up until the single cell is captured at the cell retrieving hole;
if a residual volume of the third reagent after the backwashing exceeds 1 microliter, discharging the residual volume up until the residual volume does not exceed 1 microliter; and
opening a plug of the cell retrieving hole,
wherein the single cell is not subjected to damage during the physicochemical treatment.
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