US 12,233,416 B2
Limit size lipid nanoparticles and related methods
Pieter R. Cullis, Vancouver (CA); Igor V. Jigaltsev, Vancouver (CA); Robert James Taylor, Vancouver (CA); Timothy Leaver, Delta (CA); Andre Wild, Vancouver (CA); and Nathan Maurice Belliveau, Weymouth (CA)
Assigned to The University of British Columbia, Vancouver (CA)
Filed by The University of British Columbia, Vancouver (CA)
Filed on Apr. 6, 2023, as Appl. No. 18/296,954.
Application 18/296,954 is a continuation of application No. 17/061,247, filed on Oct. 1, 2020, granted, now 11,648,556.
Application 17/061,247 is a continuation of application No. 15/927,925, filed on Mar. 21, 2018, granted, now 10,843,194, issued on Nov. 24, 2020.
Application 15/927,925 is a continuation of application No. 15/087,721, filed on Mar. 31, 2016, granted, now 9,943,846, issued on Apr. 17, 2018.
Application 15/087,721 is a continuation of application No. 14/353,460, abandoned, previously published as PCT/CA2012/000991, filed on Oct. 25, 2012.
Claims priority of provisional application 61/551,366, filed on Oct. 25, 2011.
Prior Publication US 2023/0256436 A1, Aug. 17, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 9/16 (2006.01); A61J 3/00 (2006.01); A61K 9/107 (2006.01); A61K 9/127 (2006.01); A61K 9/1271 (2025.01); A61K 9/1277 (2025.01); A61K 9/14 (2006.01); A61K 31/704 (2006.01); A61K 47/44 (2017.01); A61K 49/00 (2006.01); B01F 23/41 (2022.01); B01F 25/431 (2022.01); B01F 25/433 (2022.01); B01F 33/30 (2022.01); B01L 3/00 (2006.01)
CPC B01L 3/50273 (2013.01) [A61J 3/00 (2013.01); A61K 9/1075 (2013.01); A61K 9/127 (2013.01); A61K 9/1271 (2013.01); A61K 9/1277 (2013.01); A61K 9/14 (2013.01); A61K 31/704 (2013.01); A61K 47/44 (2013.01); A61K 49/0002 (2013.01); B01F 23/41 (2022.01); B01F 25/4317 (2022.01); B01F 25/4331 (2022.01); B01F 33/30 (2022.01); B01L 3/502715 (2013.01); B01F 25/431971 (2022.01); B01L 2200/0605 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0848 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/18 (2013.01); Y10T 428/2982 (2015.01)] 19 Claims
 
1. A device for producing limit size lipid nanoparticles having a diameter from 10 nm to 50 nm, comprising:
(a) a first inlet for receiving a first solution comprising a first solvent;
(b) a first inlet microchannel in fluid communication with the first inlet to provide a first stream comprising the first solvent;
(c) a second inlet for receiving a second solution comprising lipid particle-forming materials in a second solvent;
(d) a second inlet microchannel in fluid communication with the second inlet to provide a second stream comprising the lipid particle-forming materials in the second solvent; and
(e) a third microchannel for receiving the first and second streams, wherein the third microchannel has a first region adapted for flowing the first and second streams and a second region adapted for mixing the contents of the first and second streams to provide a third stream comprising limit size lipid nanoparticles,
wherein the third microchannel is configured to receive the first and second streams at a flow rate ratio greater than 1.0, and wherein the second region is configured to provide a fluid flow rate from 1 ml/min to 40 ml/min.