US 12,233,415 B2
Microfluidic reaction chamber for amplification of nucleic acids
Si-lam Choy, Corvallis, OR (US); Hilary Ely, Corvallis, OR (US); and John Lahmann, Corvallis, OR (US)
Assigned to Hewlett-Packard Development Company, L.P., Spring, TX (US)
Appl. No. 17/417,306
Filed by Hewlett-Packard Development Company, L.P., Spring, TX (US)
PCT Filed Apr. 29, 2019, PCT No. PCT/US2019/029707
§ 371(c)(1), (2) Date Jun. 22, 2021,
PCT Pub. No. WO2020/222763, PCT Pub. Date Nov. 5, 2020.
Prior Publication US 2022/0048026 A1, Feb. 17, 2022
Int. Cl. B01L 3/00 (2006.01); A61L 27/16 (2006.01); A61L 27/56 (2006.01); B01F 23/00 (2022.01); B01F 23/41 (2022.01); B01F 101/23 (2022.01); B01L 7/00 (2006.01); B01L 9/00 (2006.01); B23Q 17/24 (2006.01); C07K 14/705 (2006.01); C08F 220/56 (2006.01); C08L 33/26 (2006.01); C12M 1/34 (2006.01); C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); C12Q 1/6844 (2018.01); C12Q 1/686 (2018.01); G01N 1/31 (2006.01); G01N 21/17 (2006.01); G01N 21/3577 (2014.01); G01N 21/359 (2014.01); G01N 21/39 (2006.01); G01N 21/45 (2006.01); G01N 21/64 (2006.01); G01N 21/77 (2006.01); G01N 21/78 (2006.01); G01N 27/414 (2006.01); G01N 30/12 (2006.01); G01N 30/68 (2006.01); G01N 30/70 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01); G01N 33/00 (2006.01); G01N 33/18 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01); G01N 33/74 (2006.01); G01N 35/00 (2006.01); G01N 35/10 (2006.01); G06T 7/00 (2017.01); G06T 7/90 (2017.01); H10K 10/46 (2023.01); H10K 85/00 (2023.01); H10K 85/20 (2023.01)
CPC B01L 3/50273 (2013.01) [B01L 3/502761 (2013.01); B01L 7/52 (2013.01); C12Q 1/6844 (2013.01); B01L 2200/0621 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0442 (2013.01); B01L 2400/0633 (2013.01); B01L 2400/0694 (2013.01)] 6 Claims
OG exemplary drawing
 
1. An apparatus comprising:
a microfluidic reaction chamber including a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids included in the biologic sample;
a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber;
each of a plurality of valves respectively disposed in different ones of the plurality of capillaries;
a valve control system to selectively control each of the plurality of valves and, during operation, to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber according to a particular timing sequence; and
a trapping region disposed in the microfluidic reaction chamber to secure the nucleic acids in the microfluidic reaction chamber for the amplification of the nucleic acids using the reaction-chamber circuit; and
a pump disposed on a side of the microfluidic reaction chamber, wherein the pump is to terminate a flow of a reconstituted reagent solution from a second fluid chamber to the microfluidic reaction chamber when a level of the reconstituted reagent solution in the microfluidic reaction chamber reaches a threshold level.