	US 12,233,407 B2
	Particle-drop structures and methods for making and using the same
Dino Di Carlo, Los Angeles, CA (US); and Chueh-Yu Wu, Los Angeles, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed on Jan. 30, 2023, as Appl. No. 18/161,322.
Application 18/161,322 is a continuation of application No. 16/550,105, filed on Aug. 23, 2019, granted, now 11,590,489.
Application 16/550,105 is a continuation in part of application No. PCT/US2018/019486, filed on Feb. 23, 2018.
Claims priority of provisional application 62/463,272, filed on Feb. 24, 2017.
Prior Publication US 2023/0173479 A1, Jun. 8, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/02 (2006.01); B01L 3/00 (2006.01); C12N 1/04 (2006.01); C12N 5/071 (2010.01); C12N 5/0783 (2010.01); C12Q 1/6806 (2018.01); G01N 33/543 (2006.01)

CPC B01L 3/0262 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502784 (2013.01); C12N 1/04 (2013.01); C12N 5/0625 (2013.01); C12N 5/0636 (2013.01); C12Q 1/6806 (2013.01); G01N 33/5432 (2013.01); B01L 2300/021 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0896 (2013.01)]

23 Claims

1. A particle system for capturing secreted molecules from a cell, the particle system comprising one or more microparticles having: (1) a molecular capture region disposed on a surface of the one or more microparticles that is exposed to an external environment of the one or more microparticles and comprising a secretion capture moiety with affinity to the secreted molecules secreted from the cell; and (2) a cell capture region disposed on the one or more microparticles and comprising an affinity reagent configured to bind to the cell.