US 12,233,356 B2
Process for preparing extracellular vesicles
Aaron Noyes, Melrose, MA (US); Michael Doherty, Somerville, MA (US); Kimberly Ellis, Somerville, MA (US); Raymond Bourdeau, Watertown, MA (US); and Kayla Desanty, Bedford, MA (US)
Assigned to LONZA SALES AG, Basel (CH)
Appl. No. 17/593,613
Filed by LONZA SALES AG, Basel (CH)
PCT Filed Mar. 20, 2020, PCT No. PCT/US2020/024038
§ 371(c)(1), (2) Date Sep. 21, 2021,
PCT Pub. No. WO2020/191369, PCT Pub. Date Sep. 24, 2020.
Claims priority of provisional application 62/984,141, filed on Mar. 2, 2020.
Claims priority of provisional application 62/946,895, filed on Dec. 11, 2019.
Claims priority of provisional application 62/903,524, filed on Sep. 20, 2019.
Claims priority of provisional application 62/835,436, filed on Apr. 17, 2019.
Claims priority of provisional application 62/822,013, filed on Mar. 21, 2019.
Prior Publication US 2022/0387906 A1, Dec. 8, 2022
Int. Cl. B01D 15/36 (2006.01); A61K 39/00 (2006.01); C12M 1/00 (2006.01)
CPC B01D 15/362 (2013.01) [A61K 39/00 (2013.01); B01D 15/363 (2013.01); C12M 29/10 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method of preparing EVs comprising:
(i) subjecting a sample comprising the EVs to a depth filtration (depth-filtration processed filtrate sample);
(ii) contacting the depth-filtration processed filtrate sample to a nuclease (nuclease processed sample);
iii) contacting the nuclease processed sample to ultrafiltration and/or diafiltration (UF/DF processed filtrate sample);
(iv) contacting the UF/DF processed filtrate sample with a cation exchange chromatography (CEX) resin (CEX-processed sample);
(v) contacting the CEX-processed sample with an anion exchange chromatography (AEX) resin (AEX-processed sample);
(vi) contacting the AEX-processed sample with mixed-mode chromatography (MMC) resin (MMC processed sample); and
(vii) subjecting the MMC processed sample to a UF/DF.