US 12,233,188 B2
Bone marrow adipose portion isolation device and methods
Christopher Centeno, Broomfield, CO (US); Ryan Dregalla, Broomfield, CO (US); Nicolette Lyons, Broomfield, CO (US); and Patrick Reischling, Broomfield, CO (US)
Assigned to Regenexx, LLC, Des Moines, IA (US)
Filed by Regenexx, LLC, Des Moines, IA (US)
Filed on Jan. 6, 2022, as Appl. No. 17/569,738.
Application 15/958,940 is a division of application No. 14/778,530, granted, now 9,976,115, issued on May 22, 2018, previously published as PCT/US2014/049992, filed on Aug. 6, 2014.
Application 17/569,738 is a continuation of application No. 16/730,408, filed on Dec. 30, 2019, abandoned.
Application 16/730,408 is a continuation of application No. 15/958,940, filed on Apr. 20, 2018, granted, now 10,537,596, issued on Jan. 21, 2020.
Claims priority of provisional application 61/862,837, filed on Aug. 6, 2013.
Prior Publication US 2022/0184135 A1, Jun. 16, 2022
Int. Cl. A61M 1/02 (2006.01); A61K 35/28 (2015.01); A61M 1/36 (2006.01); B01D 21/26 (2006.01); B01L 3/00 (2006.01); B04B 7/08 (2006.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01); C12N 5/077 (2010.01); C12N 5/0775 (2010.01); G01N 33/49 (2006.01); G01N 33/68 (2006.01)
CPC A61M 1/029 (2013.01) [A61K 35/28 (2013.01); A61M 1/3693 (2013.01); B01D 21/26 (2013.01); B01D 21/262 (2013.01); B01L 3/50215 (2013.01); B04B 7/08 (2013.01); C12M 33/04 (2013.01); C12M 45/04 (2013.01); C12N 5/0663 (2013.01); C12N 5/0667 (2013.01); C12N 5/0669 (2013.01); G01N 33/491 (2013.01); G01N 33/6872 (2013.01); A61M 2202/08 (2013.01); A61M 2202/10 (2013.01); B01L 2200/025 (2013.01); B01L 2300/044 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0864 (2013.01); B01L 2400/0409 (2013.01)] 14 Claims
OG exemplary drawing
 
10. A method for processing a fluid comprising:
providing a device, the device comprising;
a first chamber comprising a first sidewall, and a first upper opening;
a second chamber adjacent to the first chamber, the second chamber comprising a second sidewall, and a second upper opening;
an opening through the first sidewall and second sidewall, said opening defining a fluid pathway from the first chamber to the second chamber; and
a mechanical emulsification system in fluid communication with the second chamber for emulsifying a liquid contained in the second chamber,
wherein a portion of the first sidewall and a portion of the second sidewall define a shared sidewall, wherein a first surface of the shared sidewall is a vertical inner surface of the first chamber and a second surface of the shared sidewall is a vertical inner surface of the second chamber,
wherein the opening extends through the shared sidewall from the first surface to the second surface;
placing the fluid into the first chamber;
centrifuging the fluid in the first chamber to separate the fluid into fluid fractions;
decanting one or more fluid fractions from the first chamber into the second chamber through the opening while leaving one or more fluid fractions in the first chamber;
re-centrifuging the fluid fractions in the first and second chambers; and
removing one or more fluid fractions from at least one of the first chamber and the second chamber.