US 12,233,117 B2
Immunogenic compositions, antigen screening methods, and methods of generating immune responses
Sean C. Murphy, Seattle, WA (US); and Bradley C. Stone, Seattle, WA (US)
Assigned to UNIVERSITY OF WASHINGTON, Seattle, WA (US)
Filed by UNIVERSITY OF WASHINGTON, Seattle, WA (US)
Filed on Jul. 26, 2023, as Appl. No. 18/359,740.
Application 18/359,740 is a continuation of application No. 17/165,867, filed on Feb. 2, 2021, granted, now 11,738,073.
Application 17/165,867 is a continuation of application No. 15/749,928, granted, now 10,925,950, issued on Feb. 2, 2018, previously published as PCT/US2016/045439, filed on Aug. 3, 2016.
Claims priority of provisional application 62/200,487, filed on Aug. 3, 2015.
Prior Publication US 2024/0197849 A1, Jun. 20, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/015 (2006.01); A61K 45/06 (2006.01); A61K 47/69 (2017.01); A61P 33/06 (2006.01); C07K 14/15 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/015 (2013.01) [A61K 45/06 (2013.01); A61K 47/6921 (2017.08); A61P 33/06 (2018.01); C07K 14/15 (2013.01); A61K 2039/5154 (2013.01); A61K 2039/53 (2013.01); A61K 2039/545 (2013.01); Y02A 50/30 (2018.01)] 5 Claims
 
1. An immunogenic composition comprising:
two or more plasmid pools, including a first plasmid pool and a second plasmid pool;
where at least the first plasmid pool is loaded onto or into a first aliquot of gold beads and where at least the second plasmid pool is loaded onto or into a second aliquot of gold beads;
where each of the first and the second aliquots of gold beads are loaded into a dosing container;
where the first plasmid pool comprises a first plurality of plasmids and where the second plasmid pool comprises a second plurality of plasmids different from the first plurality of plasmids;
where each plasmid in the two or more plasmid pools contains a minigene encoding an epitope or functional portion thereof from one or more proteins; and
where at least the first and the second plasmid pools in the dosing container together contain more than one of the minigenes that encode the epitope or functional portion thereof.