US 12,233,099 B2
Composition for promoting myogenesis, containing processed ginseng extract
Sun Kyu Park, Seongnam-si (KR); Jeom Yong Kim, Seongnam-si (KR); Young Hyo Yoo, Seongnam-si (KR); Min Jung Jang, Seongnam-si (KR); Chang Taek Oh, Seongnam-si (KR); Min Ju Lim, Seongnam-si (KR); Gwan Su Yi, Daejeon (KR); Yi Li, Daejeon (KR); Yoon Hyeok Lee, Seoul (KR); and Jae Cheal Yoo, Jinju-si (KR)
Assigned to GREEN CROSS WELLBEING CORPORATION, Seoul (KR)
Appl. No. 17/059,122
Filed by GREEN CROSS WELLBEING CORPORATION, Seongnam-si (KR)
PCT Filed May 24, 2019, PCT No. PCT/KR2019/006265
§ 371(c)(1), (2) Date Nov. 25, 2020,
PCT Pub. No. WO2019/226015, PCT Pub. Date Nov. 28, 2019.
Claims priority of application No. 10-2018-0059749 (KR), filed on May 25, 2018.
Prior Publication US 2021/0205387 A1, Jul. 8, 2021
Int. Cl. A61K 36/00 (2006.01); A23L 33/00 (2016.01); A23L 33/105 (2016.01); A61K 9/00 (2006.01); A61K 31/704 (2006.01); A61K 36/258 (2006.01); A61P 21/00 (2006.01)
CPC A61K 36/258 (2013.01) [A23L 33/105 (2016.08); A23L 33/40 (2016.08); A61K 9/0056 (2013.01); A61K 31/704 (2013.01); A61P 21/00 (2018.01); A23V 2002/00 (2013.01); A61K 2236/19 (2013.01); A61K 2236/51 (2013.01)] 2 Claims
 
1. A method for treating a muscle-atrophy-related disease in a mammal in need thereof, comprising administering to the mammal an extract of processed ginseng, wherein the muscle atrophy-related disease is selected from the group consisting of muscular atrophy, myopathy, muscular injury, muscular dystrophy, myasthenia, myoneural conductive disease, dermatomyositis, diabetic amyotrophy, nerve injury, amyotrophic lateral sclerosis (ALS), cachexia, and degenerative muscle diseases,
wherein the extract of processed ginseng inhibits myoatrophy caused by myostatin,
wherein the extract of processed ginseng is prepared by:
(a) seeding Aspergillus niger strain on a medium containing a ginseng powder and bran;
(b) culturing the strain in step (a);
(c) purifying the culture in step (b) through an ultrafiltration membrane;
(d) separating an enzyme from the purification product in step (c);
(e) adding the enzyme in step (d) to a ginseng powder, a red ginseng powder, a ginseng extract, or a red ginseng extract;
(f) fermenting the product after addition in step (e);
(g) separating the fermentation product in step (f);
(h) concentrating a supernatant in step (g);
(i) reacting the concentration product in step (h) with at least one organic acid selected from the group consisting of acetic acid, lactic acid, citric acid, malic acid, and tartaric acid; and
(j) neutralizing, filtering, purifying, concentrating, and drying the reaction product in step (i), and
wherein the extract of processed ginseng contains ginsenosides Rh2 and Rg3 each in 0.5-30 wt %.