US 12,233,098 B2
Preparation method of Antrodia cinnamomea water-insoluble dietary fiber
Lianzhong Ai, Shanghai (CN); Yongjun Xia, Shanghai (CN); Peng Meng, Shanghai (CN); Hui Zhang, Shanghai (CN); Guangqiang Wang, Shanghai (CN); Zhiqiang Xiong, Shanghai (CN); and Xin Song, Shanghai (CN)
Assigned to UNIVERSITY OF SHANGHAI FOR SCIENCE AND TECHNOLOGY, Shanghai (CN)
Appl. No. 17/431,145
Filed by UNIVERSITY OF SHANGHAI FOR SCIENCE AND TECHNOLOGY, Shanghai (CN)
PCT Filed Sep. 11, 2020, PCT No. PCT/CN2020/114638
§ 371(c)(1), (2) Date Aug. 13, 2021,
PCT Pub. No. WO2021/174801, PCT Pub. Date Sep. 10, 2021.
Claims priority of application No. 202010135376 (CN), filed on Mar. 2, 2020.
Prior Publication US 2022/0305069 A1, Sep. 29, 2022
Int. Cl. A61K 36/07 (2006.01); A23L 31/00 (2016.01); A23L 33/21 (2016.01); A61P 3/06 (2006.01); B01D 11/02 (2006.01)
CPC A61K 36/07 (2013.01) [A23L 31/00 (2016.08); A23L 33/21 (2016.08); A61P 3/06 (2018.01); B01D 11/0288 (2013.01); A23V 2002/00 (2013.01); A61K 2236/331 (2013.01); A61K 2236/333 (2013.01); A61K 2236/39 (2013.01); A61K 2236/51 (2013.01)] 7 Claims
 
1. A preparation method of Antrodia cinnamomea water-insoluble dietary fiber, comprising:
extracting ethanol and water from a fruit body or mycelium of Antrodia cinnamomea to obtain Antrodia cinnamomea wastes, which are residues remaining after the ethanol and water extraction, the Antrodia cinnamomea wastes are dried and comminuted to obtain an Antrodia cinnamomea waste powder;
treating the Antrodia cinnamomea waste powder with an NaOH solution containing NaBH4 and extracting twice therefrom to obtain two extracts with a material-to-liquid ratio of 1:5-1:25 v/m;
combining the two extracts and adding a glacial acetic acid for neutralization, after a first predetermined time, centrifugalizing the extracts to collect a precipitate;
washing the precipitate three times with water and dissolving the precipitate in a LiCl-DMSO solution, the precipitate that is insoluble in the LiCl-DMSO solution is dialyzed for a second predetermined time and freeze-dried to obtain a water-insoluble dietary fiber component of Antrodia cinnamomea that is insoluble in DMSO referred to as ACA-IDK; and
subjecting the LiCl-DMSO solution to an ethanol/DMSO fractional precipitation, an ethanol addition ratio being 30%-90% v/v, then collecting the precipitate, dialyzing the precipitate for a third predetermined time, and freeze-drying the precipitate to obtain the water-insoluble dietary fiber component of Antrodia cinnamomea that is soluble in DMSO referred to as ACA-DK.