US 12,233,078 B2
Method and kit for analysis of drug resistance of tumor cells
Hongjing Dou, Shanghai (CN); and Lingshan Liu, Shanghai (CN)
Assigned to Shanghai Jiao Tong University, Shanghai (CN)
Filed by Shanghai Jiao Tong University, Shanghai (CN)
Filed on Jun. 10, 2022, as Appl. No. 17/837,417.
Claims priority of application No. 202110654859.4 (CN), filed on Jun. 11, 2021.
Prior Publication US 2022/0395518 A1, Dec. 15, 2022
Int. Cl. A61K 31/695 (2006.01); A61K 9/14 (2006.01); A61K 47/69 (2017.01); G01N 33/574 (2006.01); G01N 21/01 (2006.01); G01N 21/64 (2006.01); G01N 33/50 (2006.01)
CPC A61K 31/695 (2013.01) [A61K 9/14 (2013.01); A61K 47/6933 (2017.08); G01N 33/574 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A method for analysis of drug resistance of tumor cells, comprising the steps of:
(a) providing silicon dioxide nanoparticles, polystyrene-co-polyacrylic acid nanoparticles or metal-organic framework nanoparticles;
(b) co-incubating the silicon dioxide nanoparticles, the polystyrene-co-polyacrylic acid nanoparticles or the metal-organic framework nanoparticles with the tumor cells; and
(c) detecting endocytosis of the silicon dioxide nanoparticles, the polystyrene-co-polyacrylic acid nanoparticles or the metal-organic framework nanoparticles by the tumor cells;
wherein the tumor cells comprise drug-resistant tumor cells and/or non-drug-resistant tumor cells;
the drug-resistant tumor cells include at least one of paclitaxel-resistant human lung cancer (A549) cells, paclitaxel-resistant human leukemia (K562) cells, adriamycin-resistant human leukemia (K562) cells, paclitaxel-resistant human breast cancer (MCF-7) cells, vincristine-resistant human colon cancer (HCT-8) cells, paclitaxel-resistant human ovarian cancer (A2780) cells, cisplatin-resistant human ovarian cancer (SKOV3) cells, paclitaxel-resistant human cervical cancer (Hela) cells, and paclitaxel-resistant human ovarian cancer (SKOV3) cells;
the non-drug-resistant tumor cells include at least one of human lung cancer (A549) cells, human leukemia (K562) cells, human breast cancer (MCF-7) cells, human colon cancer (HCT-8) cells, human ovarian cancer (A2780) cells, human ovarian cancer (SKOV3) cells and human cervical cancer (Hela) cells.