| CPC A61B 5/14555 (2013.01) [A61B 3/0025 (2013.01); A61B 3/12 (2013.01); A61B 3/14 (2013.01); A61B 5/7267 (2013.01); A61B 5/7278 (2013.01)] | 7 Claims |
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1. An imaging method using a multi-spectral fundus imaging system using a dynamic visual stimulation, comprising:
1) acquiring a multi-spectral image before applying optical stimulation:
a) monitoring mode:
a camera first works in an infrared light monitoring mode after upon start-up, adjusting a relative position of the multi-spectral fundus imaging system and a fundus of a subject, and adjusting a focusing of the camera to find an area of the fundus of the subject;
b) external trigger mode:
after finding the area of the fundus of the subject, the camera works in an external or internal trigger mode, and a computer controls a multi-spectral light source to synchronize with the camera; monochromatic excitation light emitted by the multi-spectral light source passes through a multi-in-one ring-shaped optical fiber bundle and is output from a ring-shaped light outlet to form a ring-shaped light; the ring-shaped light being focused and changed by a first focusing lens and light path adjustment device; and reflected around a central hole of a mid-pass mirror; the ring-shaped light entering into the fundus after being focused by a second focusing lens; reflected imaging light from the fundus is focused by the second focusing lens to the central hole of the mid-pass mirror and passes through the central hole; then passing through an imaging focusing lens group and entering the camera; the computer controls the multi-spectral light source and the camera to perform static imaging to complete a multi-spectral fundus image acquisition;
c) the multi-spectral light source changes a wavelength of the excitation light and the computer controls the multi-spectral light source to synchronize with the camera; the camera simultaneously takes corresponding multi-spectral fundus images of the corresponding wavelength, and the steps a) and b) are repeated until multi-spectral fundus images corresponding to multiple different wavelengths are taken;
d) transmitting to the computer to form a set of multi-spectral images;
2) applying optical stimulation:
a) adjusting a dichroic mirror to enter a light path; and the computer controls an optical stimulation device to send a pattern, which is reflected by the dichroic mirror, and then passes through the imaging focusing lens group, the central hole of the mid-pass mirror, and the second focusing lens in sequence, and transmits to the fundus; a contrast between light and dark in the pattern corresponds to a strength of an optical stimulation to the fundus when the dynamic visual stimulation of the fundus is regulated;
b) after the optical stimulation ends, turning off the optical stimulation device and moving the dichroic mirror out of the light path;
3) acquiring multiple sets of multi-spectral images in time series after applying optical stimulation:
according to the method in step 1), performing multi-spectral image acquisition at different time points on the fundus after optical stimulation by the camera, and transmitting it to the computer; and
calculating a blood oxygen saturation value:
4) constituting functional imaging by multiple multi-spectral imaging at different time points before and after applying optical stimulation, and calculating a blood oxygen saturation value by a computer according to the multiple multi-spectral images at different time points before and after the applying of optical stimulation, and monitoring a dynamic blood oxygen saturation value to evaluate dynamic response and recovery of a fundus circulation and nervous system to optical stimulation.
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