US 12,232,497 B2
Mixed bacteria for promoting nodulation and nitrogen fixation of Robinia pseudoacacia and application thereof
Jiayao Zhuang, Nanjing (CN); Chao Liu, Nanjing (CN); Xiaoxue Wang, Nanjing (CN); Jiaxin Zheng, Nanjing (CN); and Kun Tian, Nanjing (CN)
Assigned to NANJING FORESTRY UNIVERSITY, Nanjing (CN)
Filed by NANJING FORESTRY UNIVERSITY, Nanjing (CN)
Filed on Jan. 12, 2021, as Appl. No. 17/147,173.
Application 17/147,173 is a continuation of application No. PCT/CN2020/113434, filed on Sep. 4, 2020.
Claims priority of application No. 2019109381957 (CN), filed on Sep. 29, 2019.
Prior Publication US 2024/0196904 A1, Jun. 20, 2024
Int. Cl. A01N 63/22 (2020.01); A01N 25/02 (2006.01); C12N 1/20 (2006.01); C12R 1/07 (2006.01)
CPC A01N 63/22 (2020.01) [A01N 25/02 (2013.01); C12N 1/205 (2021.05); C12N 2500/12 (2013.01); C12N 2500/74 (2013.01); C12N 2501/998 (2013.01); C12N 2523/00 (2013.01); C12N 2527/00 (2013.01); C12R 2001/07 (2021.05)] 8 Claims
OG exemplary drawing
 
1. A method for promoting nodulation and nitrogen fixation of Robinia pseudoacacia Robinia pseudoacacia comprising a step of adding a mixed bacteria to a Robinia pseudoacacia seedling, wherein the mixed bacteria comprises Kocuria sp. X-22, Microbacterium sp. X-26, and Bacillus sp. X-28; and
wherein the Kocuria sp. X-22 is preserved in China Center for Type Culture Collection with a preservation date of Apr. 8, 2019 and a preservation number of CCTCC No: M 2019237; the preservation address is Wuhan University, Wuhan, China;
the Microbacterium sp. X-26 is preserved in China Center for Type Culture Collection with a preservation date of Apr. 8, 2019 and a preservation number of CCTCC No: M 2019238; the preservation address is Wuhan University, Wuhan, China; and
the Bacillus sp. X-28 is preserved in China Center for Type Culture Collection with a preservation date of Apr. 8, 2019 and the preservation number of CCTCC No: M 2019239; the preservation address is Wuhan University, Wuhan, China.