US 12,232,496 B2
Methods and system for stimulating root exudation in plants
Asaph Aharoni, Tel Aviv (IL); and Elisa Korenblum, Rehovot (IL)
Assigned to YEDA RESEARCH AND DEVELOPMENT CO. LTD, Rehovot (IL)
Appl. No. 17/040,227
Filed by YEDA RESEARCH AND DEVELOPMENT CO. LTD, Rehovot (IL)
PCT Filed Mar. 21, 2019, PCT No. PCT/IL2019/050322
§ 371(c)(1), (2) Date Sep. 22, 2020,
PCT Pub. No. WO2019/180722, PCT Pub. Date Sep. 26, 2019.
Claims priority of application No. 258319 (IL), filed on Mar. 22, 2018.
Prior Publication US 2021/0007363 A1, Jan. 14, 2021
Int. Cl. A01N 63/20 (2020.01); A01G 29/00 (2006.01); A01N 63/38 (2020.01); A61K 36/185 (2006.01); A61K 36/81 (2006.01); C07D 305/14 (2006.01); C07J 17/00 (2006.01)
CPC A01N 63/20 (2020.01) [A01G 29/00 (2013.01); A01N 63/38 (2020.01); A61K 36/185 (2013.01); A61K 36/81 (2013.01); C07D 305/14 (2013.01); C07J 17/005 (2013.01); A61K 2236/00 (2013.01)] 3 Claims
 
1. A method for obtaining an exudate from a Cannabis sativa root, the method comprising:
a) providing Cannabis sativa;
b) splitting a root of the Cannabis sativa into at least two root moieties;
c) placing each root moiety of the Cannabis sativa into a separate container or compartment;
d) stimulating a first root moiety of the Cannabis sativa or an aerial portion of the Cannabis sativa with methyl jasmonate to induce exudation or secretion of an exudate by the second root moiety of the Cannabis sativa into the container or compartment of the second root moiety; and
e) harvesting the exudate from the container or compartment wherein said exudate comprises a metabolite of interest, wherein said metabolite of interest comprises a cannabinoid, a terpenoid, or a combination thereof.