US 12,232,494 B2
Aqueous compositions comprising solubilzed lipo-chitooligosaccharides
Kenneth Kellar, Fuquay-Varina, NC (US)
Assigned to NOVOZYMES BIOAG A/S, Bagsvaerd (DK)
Appl. No. 16/960,499
Filed by NOVOZYMES BIOAG A/S, Bagsvaerd (DK)
PCT Filed Jan. 4, 2019, PCT No. PCT/US2019/012259
§ 371(c)(1), (2) Date Jul. 7, 2020,
PCT Pub. No. WO2019/136198, PCT Pub. Date Jul. 11, 2019.
Claims priority of provisional application 62/614,544, filed on Jan. 8, 2018.
Prior Publication US 2020/0375179 A1, Dec. 3, 2020
Int. Cl. A01N 43/16 (2006.01); A01N 25/04 (2006.01); A01N 25/30 (2006.01); A01N 63/20 (2020.01)
CPC A01N 43/16 (2013.01) [A01N 25/04 (2013.01); A01N 25/30 (2013.01); A01N 63/20 (2020.01)] 25 Claims
 
1. A method of solubilizing lipo-chitooligosaccharide (LCO) molecules in an aqueous solvent, said method comprising:
contacting said LCO molecules with an anionic surfactant comprising a carbonate, phosphate, sulfate, or sulfonate head and a linear hydrocarbon tail that is at least 8 carbons in length in an amount/concentration sufficient to reduce and/or prevent agglomeration of said LCO molecules; and
contacting said LCO molecules with a nonionic surfactant comprising a hydrocarbon chain and an ethoxylate chain in an amount/concentration sufficient to form micelles comprising said LCO molecules,
wherein at least 75% of said LCO molecules are solubilized within micelles capable of passing through a filter having an average pore size of 0.22 microns.
 
5. An aqueous lipo-chitooligosaccharide (LCO) solution, comprising:
an aqueous solvent;
LCO molecules;
an anionic surfactant comprising a carbonate, phosphate, sulfate, or sulfonate head and a linear hydrocarbon tail that is at least 8 carbons in length, said anionic surfactant present in an amount/concentration sufficient to reduce and/or prevent agglomeration of said LCO molecules; and
a nonionic surfactant present comprising a hydrocarbon chain and an ethoxylate chain, said nonionic surfactant present in an amount/concentration sufficient to form micelles comprising at least a portion of said LCO molecules,
wherein at least 75% of said LCO molecules are solubilized within micelles capable of passing through a filter having an average pore size of 0.22 microns.