US 11,905,539 B2
Isopropylmalate synthase variant and a method of producing L-leucine using the same
Ji Hye Lee, Gyeonggi-do (KR); Byeong Cheol Song, Gyeonggi-do (KR); Ae Ji Jeon, Seoul (KR); Jong Hyun Kim, Gyeonggi-do (KR); and Hye Won Kim, Gyeonggi-do (KR)
Assigned to CJ Cheiljedang Corporation, Seoul (KR)
Filed by CJ Cheiljedang Corporation, Seoul (KR)
Filed on Jan. 25, 2021, as Appl. No. 17/156,998.
Application 17/156,998 is a continuation of application No. 16/473,941, granted, now 11,104,924, previously published as PCT/KR2017/011622, filed on Oct. 20, 2017.
Claims priority of application No. 10-2016-0181343 (KR), filed on Dec. 28, 2016.
Prior Publication US 2021/0254111 A1, Aug. 19, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 13/06 (2006.01); C12N 9/10 (2006.01); C12N 15/52 (2006.01); C12N 15/77 (2006.01); C12N 15/74 (2006.01)
CPC C12P 13/06 (2013.01) [C12N 9/1025 (2013.01); C12N 15/52 (2013.01); C12N 15/74 (2013.01); C12N 15/77 (2013.01); C12Y 203/03013 (2013.01)] 10 Claims
 
1. A modified microorganism belonging to the genus of Corynebacterium for producing L-leucine, comprising a modified polypeptide having an isopropylmalate synthase activity,
wherein glycine at position 561 from a N-terminus of a polypeptide consisting of the amino acid sequence of SEQ ID NO: 1 is substituted with aspartic acid in the modified polypeptide, and
wherein the modified microorganism has a resistance to norleucine or L-leucine, and
wherein a native promoter of a polynucleotide encoding the modified polypeptide in the microorganism is substituted with a promoter which enhances an expression of the polynucleotide compared to the native promoter.