CPC C12P 13/06 (2013.01) [C12N 9/1025 (2013.01); C12N 15/52 (2013.01); C12N 15/74 (2013.01); C12N 15/77 (2013.01); C12Y 203/03013 (2013.01)] | 10 Claims |
1. A modified microorganism belonging to the genus of Corynebacterium for producing L-leucine, comprising a modified polypeptide having an isopropylmalate synthase activity,
wherein glycine at position 561 from a N-terminus of a polypeptide consisting of the amino acid sequence of SEQ ID NO: 1 is substituted with aspartic acid in the modified polypeptide, and
wherein the modified microorganism has a resistance to norleucine or L-leucine, and
wherein a native promoter of a polynucleotide encoding the modified polypeptide in the microorganism is substituted with a promoter which enhances an expression of the polynucleotide compared to the native promoter.
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