CPC C12N 15/1065 (2013.01) [C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6881 (2013.01); C12Q 1/6883 (2013.01); G16B 40/00 (2019.02); C12Q 2600/16 (2013.01)] | 3 Claims |
1. A method of providing diagnostic assessment of immunocompetence in a solid organ transplant recipient undergoing immune suppressive therapy, comprising:
(a) identifying a T cell repertoire in the recipient in vitro;
(b) determining the diversity of said T cell repertoire identified in step
(a) in a pre-transplant sample from the recipient;
(c) determining the diversity of said T cell repertoire identified in step
(a) in a post-transplant sample from the recipient;
(d) comparing the diversities of said T cell repertoire in said pre- and post-transplant samples;
(e) determining that immune reconstitution is not occurring based on the comparison of the diversities of said T cell repertoire in the post-transplant sample and the pre-transplant sample; and
(f) actively modifying treatment to withdraw immunosuppressive therapy to the recipient.
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