US 11,905,511 B2
Method of measuring adaptive immunity
Harlan S. Robins, Seattle, WA (US); Edus H. Warren, Bainbridge Island, WA (US); and Christopher Scott Carlson, Kirland, WA (US)
Assigned to Fred Hutchinson Cancer Center, Seattle, WA (US)
Filed by Fred Hutchinson Cancer Center, Seattle, WA (US)
Filed on Jun. 29, 2018, as Appl. No. 16/023,010.
Application 16/023,010 is a continuation of application No. 15/709,719, filed on Sep. 20, 2017, granted, now 11,214,793, issued on Nov. 7, 2017.
Application 15/709,719 is a continuation of application No. 15/061,827, filed on Mar. 4, 2016, granted, now 9,809,813, issued on Nov. 7, 2017.
Application 15/061,827 is a continuation of application No. 14/243,875, filed on Apr. 2, 2014, abandoned.
Application 14/243,875 is a continuation of application No. 12/794,507, filed on Jun. 4, 2010, abandoned.
Claims priority of provisional application 61/220,344, filed on Jun. 25, 2009.
Prior Publication US 2018/0312832 A1, Nov. 1, 2018
Int. Cl. C12N 15/10 (2006.01); G16B 40/00 (2019.01); C12Q 1/6883 (2018.01); C12Q 1/6874 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6881 (2018.01)
CPC C12N 15/1065 (2013.01) [C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6881 (2013.01); C12Q 1/6883 (2013.01); G16B 40/00 (2019.02); C12Q 2600/16 (2013.01)] 3 Claims
 
1. A method of providing diagnostic assessment of immunocompetence in a solid organ transplant recipient undergoing immune suppressive therapy, comprising:
(a) identifying a T cell repertoire in the recipient in vitro;
(b) determining the diversity of said T cell repertoire identified in step
(a) in a pre-transplant sample from the recipient;
(c) determining the diversity of said T cell repertoire identified in step
(a) in a post-transplant sample from the recipient;
(d) comparing the diversities of said T cell repertoire in said pre- and post-transplant samples;
(e) determining that immune reconstitution is not occurring based on the comparison of the diversities of said T cell repertoire in the post-transplant sample and the pre-transplant sample; and
(f) actively modifying treatment to withdraw immunosuppressive therapy to the recipient.