US 11,905,506 B2
Multilayered cell culture apparatus
Gregory Roger Martin, Acton, ME (US); and Allison Jean Tanner, Portsmouth, NH (US)
Assigned to CORNING INCORPORATED, Corning, NY (US)
Filed by CORNING INCORPORATED, Corning, NY (US)
Filed on Feb. 3, 2022, as Appl. No. 17/591,747.
Application 12/783,217 is a division of application No. 11/433,859, filed on May 11, 2006, granted, now 7,745,209, issued on Jun. 29, 2010.
Application 17/591,747 is a continuation of application No. 15/671,485, filed on Aug. 8, 2017, granted, now 11,274,273.
Application 15/671,485 is a continuation of application No. 14/812,671, filed on Jul. 29, 2015, granted, now 9,845,451, issued on Dec. 19, 2017.
Application 14/812,671 is a continuation of application No. 14/699,157, filed on Apr. 29, 2015, abandoned.
Application 14/699,157 is a continuation of application No. 14/075,446, filed on Nov. 8, 2013, granted, now 9,040,290, issued on May 26, 2015.
Application 14/075,446 is a continuation of application No. 13/904,171, filed on May 29, 2013, granted, now 8,846,399, issued on Sep. 30, 2014.
Application 13/904,171 is a continuation of application No. 13/591,566, filed on Aug. 22, 2012, granted, now 8,470,589, issued on Jun. 25, 2013.
Application 13/591,566 is a continuation of application No. 12/783,217, filed on May 19, 2010, granted, now 8,273,572, issued on Sep. 25, 2012.
Claims priority of provisional application 60/702,896, filed on Jul. 26, 2005.
Prior Publication US 2022/0154119 A1, May 19, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/24 (2006.01); C12M 1/12 (2006.01); C12M 1/04 (2006.01); C12N 5/071 (2010.01); C12M 1/34 (2006.01)
CPC C12M 23/08 (2013.01) [C12M 23/04 (2013.01); C12M 23/24 (2013.01); C12M 23/34 (2013.01); C12M 23/38 (2013.01); C12M 23/58 (2013.01); C12M 25/02 (2013.01); C12M 25/04 (2013.01); C12M 25/06 (2013.01); C12M 29/04 (2013.01); C12M 41/00 (2013.01); C12N 5/0602 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A cell culture vessel comprising:
a single manifold;
a plurality of stacked cell growth chambers, each cell growth chamber of said plurality stacked cell growth chambers defined by a gas permeable, liquid impermeable membrane, and an opposing surface, said opposing surface being attached to said gas permeable, liquid impermeable membrane by a pair of side walls and a pair of end walls; and
supports extending between adjacent cell growth chambers of said plurality of stacked cell growth chambers,
a plurality of tracheal spaces, each tracheal space of said plurality of tracheal spaces being positioned between adjacent cell growth chambers of said plurality of stacked cell growth chambers, and being defined by each said opposing surface of one cell growth chamber and the gas permeable, liquid impermeable membrane of the adjacent cell growth chamber, each said tracheal space being in gaseous communication with the cell culture vessel's external environment, and
wherein each cell growth chamber of said plurality of stacked cell growth chambers is in fluid communication with the single manifold through one of said end walls.