CPC C12M 23/08 (2013.01) [C12M 23/04 (2013.01); C12M 23/24 (2013.01); C12M 23/34 (2013.01); C12M 23/38 (2013.01); C12M 23/58 (2013.01); C12M 25/02 (2013.01); C12M 25/04 (2013.01); C12M 25/06 (2013.01); C12M 29/04 (2013.01); C12M 41/00 (2013.01); C12N 5/0602 (2013.01)] | 13 Claims |
1. A cell culture vessel comprising:
a single manifold;
a plurality of stacked cell growth chambers, each cell growth chamber of said plurality stacked cell growth chambers defined by a gas permeable, liquid impermeable membrane, and an opposing surface, said opposing surface being attached to said gas permeable, liquid impermeable membrane by a pair of side walls and a pair of end walls; and
supports extending between adjacent cell growth chambers of said plurality of stacked cell growth chambers,
a plurality of tracheal spaces, each tracheal space of said plurality of tracheal spaces being positioned between adjacent cell growth chambers of said plurality of stacked cell growth chambers, and being defined by each said opposing surface of one cell growth chamber and the gas permeable, liquid impermeable membrane of the adjacent cell growth chamber, each said tracheal space being in gaseous communication with the cell culture vessel's external environment, and
wherein each cell growth chamber of said plurality of stacked cell growth chambers is in fluid communication with the single manifold through one of said end walls.
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