US 11,905,323 B2
Recombinant chimeric protein for selectins targeting
Federico Maisano, Lodi (IT); Federico Crivellin, Caselle Torinese (IT); Thierry Bettinger, Peillonnex (FR); Philippe Bussat, Pers-Jussy (FR); Samir Cherkaoui, Feigeres (FR); Christian Koller, Geneva (CH); and Adrian Lobito, Belmont, CA (US)
Assigned to Bracco Suisse, Manno (CH)
Filed by Bracco Suisse SA, Manno (CH)
Filed on May 18, 2022, as Appl. No. 17/747,315.
Application 17/747,315 is a division of application No. 16/076,664, granted, now 11,370,826, previously published as PCT/EP2017/052821, filed on Feb. 9, 2017.
Claims priority of application No. 16154868 (EP), filed on Feb. 9, 2016.
Prior Publication US 2022/0389080 A1, Dec. 8, 2022
Int. Cl. C07K 14/705 (2006.01); A61K 49/00 (2006.01); A61K 49/22 (2006.01); A61K 49/08 (2006.01); C12N 9/10 (2006.01); A61K 38/00 (2006.01)
CPC C07K 14/70564 (2013.01) [A61K 49/0032 (2013.01); A61K 49/0056 (2013.01); A61K 49/0084 (2013.01); A61K 49/085 (2013.01); A61K 49/221 (2013.01); A61K 49/223 (2013.01); C07K 14/705 (2013.01); C12N 9/1051 (2013.01); A61K 38/00 (2013.01); C12Y 204/01039 (2013.01); C12Y 204/01068 (2013.01); G01N 2333/70564 (2013.01)] 16 Claims
 
1. A conjugate compound comprising:
a dimeric protein comprising two recombinant chimeric P-Selectin Glycoprotein Ligand-1 (PSGL-1) proteins, wherein the monomeric recombinant chimeric PSGL-1 protein comprises at least: a selectin Binding domain able to specifically bind a selectin protein and comprising at least aa 5-16 of SEQ ID NO:11 (mature PSGL-1 sequence), a leucine zipper domain able to form a right-handed a helix thus promoting dimerization through protein-protein interaction and comprising an amino acid sequence at least 90% homologous or identical to aa 187-208 of SEQ ID NO:12 (Neural Retina-specific Leucine Zipper) and a disulfide bonds promoting region comprising at least one cysteine available to form a disulfide bond with another cysteine in a monomeric chimeric protein counterpart, so that the two chimeric protein monomers are covalently linked to each other by at least a disulfide bond; and
a diagnostic or therapeutic moiety.