US 11,905,316 B2
Applications of ZmROA1 protein in regulating plant density tolerance
Lixing Yuan, Beijing (CN); Zheng Zhao, Beijing (CN); and Kunhui He, Beijing (CN)
Assigned to CHINA AGRICULTURAL UNIVERSITY, Beijing (CN)
Filed by China Agricultural University, Beijing (CN)
Filed on May 12, 2023, as Appl. No. 18/316,371.
Claims priority of application No. 202210569763.2 (CN), filed on May 24, 2022.
Prior Publication US 2023/0382959 A1, Nov. 30, 2023
Int. Cl. C07K 14/41 (2006.01); C12N 15/82 (2006.01); C07K 14/415 (2006.01)
CPC C07K 14/415 (2013.01) [C12N 15/8262 (2013.01); C12N 15/8271 (2013.01)] 1 Claim
 
1. A method of increasing maize density tolerance, comprising a step of increasing an abundance of a ZmROA1 protein in a maize plant by overexpression of a gene encoding the ZmROA1 protein, wherein a ZmROA1 protein overexpressed has the amino acid sequence shown in SEQ ID NO: 3, and the ZmROA1protein overexpression comprises inserting a gene encoding the ZmROA1 protein comprising the nucleotide sequence shown in SEQ ID NO: 1 between two XcmI restriction sites of a pBCXUN expression vector comprising a promoter of the Ubiquitin1 gene; transforming an immature maize plant embryo; expressing the recombinant ZmROA1 gene; producing overexpression maize lines and selecting a transformed maize plant from the overexpression maize lines that exhibits a reduced root angle, improved density tolerance, reduced plant height, and reduced ear height as compared to control maize plant not comprising said overexpression of the recombinant ZmROA1 gene.