CPC C07K 14/415 (2013.01) [C12N 15/8262 (2013.01); C12N 15/8271 (2013.01)] | 1 Claim |
1. A method of increasing maize density tolerance, comprising a step of increasing an abundance of a ZmROA1 protein in a maize plant by overexpression of a gene encoding the ZmROA1 protein, wherein a ZmROA1 protein overexpressed has the amino acid sequence shown in SEQ ID NO: 3, and the ZmROA1protein overexpression comprises inserting a gene encoding the ZmROA1 protein comprising the nucleotide sequence shown in SEQ ID NO: 1 between two XcmI restriction sites of a pBCXUN expression vector comprising a promoter of the Ubiquitin1 gene; transforming an immature maize plant embryo; expressing the recombinant ZmROA1 gene; producing overexpression maize lines and selecting a transformed maize plant from the overexpression maize lines that exhibits a reduced root angle, improved density tolerance, reduced plant height, and reduced ear height as compared to control maize plant not comprising said overexpression of the recombinant ZmROA1 gene.
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