US 11,904,011 B2
North American porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof
Jay Gregory Calvert, Otsego, MI (US); David Ewell Slade, Portage, MI (US); and Siao-Kun W. Welch, Kalamazoo, MI (US)
Assigned to Zoetis Services LLC, Parsippany, NJ (US)
Filed by Zoetis Services LLC, Parsippany, NJ (US)
Filed on Jun. 6, 2022, as Appl. No. 17/832,778.
Application 17/832,778 is a continuation of application No. 16/809,038, filed on Mar. 4, 2020, granted, now 11,351,243.
Application 16/809,038 is a continuation of application No. 15/411,102, filed on Jan. 20, 2017, abandoned.
Application 15/411,102 is a continuation of application No. 13/884,077, granted, now 9,566,324, issued on Feb. 14, 2017, previously published as PCT/IB2011/055003, filed on Nov. 9, 2011.
Claims priority of provisional application 61/412,006, filed on Nov. 10, 2010.
Prior Publication US 2022/0305110 A1, Sep. 29, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/12 (2006.01); C07K 14/005 (2006.01); C12N 7/00 (2006.01); C07K 16/08 (2006.01); C12N 15/86 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/12 (2013.01) [C07K 14/005 (2013.01); C07K 16/08 (2013.01); C12N 7/00 (2013.01); C12N 15/86 (2013.01); A61K 2039/5254 (2013.01); A61K 2039/552 (2013.01); A61K 2039/57 (2013.01); C12N 2770/10022 (2013.01); C12N 2770/10034 (2013.01); C12N 2770/10043 (2013.01); C12N 2770/10051 (2013.01); C12N 2770/10062 (2013.01); C12N 2770/10064 (2013.01); C12N 2770/10071 (2013.01)] 4 Claims
 
1. A method of reducing Porcine Reproductive and Respiratory Syndrome Virus (PRRS)-induced reproductive symptoms in a sow, the method comprising administering to said sow:
a) an isolated Porcine Reproductive and Respiratory Syndrome Virus (PRRS) wherein the protein thereof encoded by ORF1a is selected from a group consisting of those amino acid sequences that contain:
i) one, two, three or four of:
an amino acid N within the amino acid sequence AMANVYD (SEQ ID NO: 9);
an amino acid N within the amino acid sequence IGHNAVM (SEQ ID NO: 12);
an amino acid Y within the amino acid sequence CVVWYLFD (SEQ ID NO: 18); and
an amino acid H within the amino acid sequence HGVHGKY (SEQ ID NO: 21); and
ii) one or both of:
an amino acid T within the amino acid sequence PSATDTS (SEQ ID NO: 27); and
an amino acid C within the amino acid sequence APMCQDE (SEQ ID NO: 33); and
iii) one or both of:
an amino acid T within the amino acid sequence CAPTGMD (SEQ ID NO: 36); and
an amino acid A within the amino acid sequence PKVAKVS (SEQ ID NO: 39); and
iv) an amino acid N within the amino acid sequence ADFNPEK (SEQ ID NO: 45); or
b) a plasm id capable of directly transfecting a suitable host cell and expressing a Porcine Reproductive and Respiratory Syndrome Virus (PRRS) from the suitable host cell so transfected, which plasm id comprises: (1) a DNA sequence encoding an infectious RNA molecule encoding the PRRS virus, and (2) a promoter capable of transcribing said infectious RNA molecule, wherein the protein encoded by ORF1a of said virus has an amino acid sequence that contains:
(i) one, two, three, or four of
an amino acid N within the amino acid sequence ANV (see SEQ ID NO: 9);
an amino acid N within the amino acid sequence HNA (see SEQ ID NO: 12);
an amino acid Y within the amino acid sequence WYL (see SEQ ID NO: 18); and
an amino acid H within the amino acid sequence VHG (see SEQ ID NO: 21); and
(ii) one or both of
an amino acid T within the amino acid sequence ATD (see SEQ ID NO: 27); and
an amino acid C within the amino acid sequence MCQ (see SEQ ID NO: 33); and
(iii) one or both of
an amino acid T within the amino acid sequence PTG (see SEQ ID NO: 36); and
an amino acid A within the amino acid sequence VAK (see SEQ ID NO: 39); and
(4) an amino acid N within the amino acid sequence FNP (see SEQ ID NO: 45).