US 11,903,972 B2
Methods and compositions for modulating peripheral immune function
Mo Dao, Huntington Beach, CA (US); and Casey C Case, San Mateo, CA (US)
Assigned to SanBio, Inc., Oakland, CA (US)
Filed by SanBio, Inc., Mountain View, CA (US)
Filed on Dec. 12, 2019, as Appl. No. 16/712,863.
Application 16/712,863 is a continuation of application No. 15/494,208, filed on Apr. 21, 2017, granted, now 10,543,234.
Application 15/494,208 is a continuation of application No. 14/298,001, filed on Jun. 6, 2014, granted, now 9,655,927, issued on May 23, 2017.
Application 14/298,001 is a continuation of application No. 13/441,311, filed on Apr. 6, 2012, granted, now 8,785,190, issued on Jul. 22, 2014.
Claims priority of provisional application 61/541,248, filed on Sep. 30, 2011.
Claims priority of provisional application 61/516,637, filed on Apr. 6, 2011.
Prior Publication US 2020/0113944 A1, Apr. 16, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 35/28 (2015.01); C12N 5/0783 (2010.01); C12N 5/0784 (2010.01); C12N 5/0786 (2010.01); C12N 5/0775 (2010.01); C12N 15/85 (2006.01)
CPC A61K 35/28 (2013.01) [C12N 5/0637 (2013.01); C12N 5/0639 (2013.01); C12N 5/0645 (2013.01); C12N 5/0663 (2013.01); C12N 15/85 (2013.01); C12N 2501/2302 (2013.01); C12N 2501/42 (2013.01); C12N 2502/1358 (2013.01)] 10 Claims
 
1. A method for suppressing the function of a TH1 helper T cell in a subject, the method comprising:
administering to the subject an effective amount of cells, wherein the cells are obtained by a method comprising:
(a) providing a culture of mesenchymal stem cells;
(b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
(c) selecting cells that comprise the polynucleotide of step (b); and
(d) further culturing the selected cells of step (c) in the absence of selection for the polynucleotide;
wherein the function is selected from one or more of:
(i) activation of macrophages,
(ii) activation of cytotoxic T-cells,
(iii) activation of B cells,
(iv) secretion of interferon gamma (IFN-γ), and
(v) secretion of tumor necrosis factor alpha (TNF-α).