CPC A61K 35/28 (2013.01) [C12N 5/0637 (2013.01); C12N 5/0639 (2013.01); C12N 5/0645 (2013.01); C12N 5/0663 (2013.01); C12N 15/85 (2013.01); C12N 2501/2302 (2013.01); C12N 2501/42 (2013.01); C12N 2502/1358 (2013.01)] | 10 Claims |
1. A method for suppressing the function of a TH1 helper T cell in a subject, the method comprising:
administering to the subject an effective amount of cells, wherein the cells are obtained by a method comprising:
(a) providing a culture of mesenchymal stem cells;
(b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
(c) selecting cells that comprise the polynucleotide of step (b); and
(d) further culturing the selected cells of step (c) in the absence of selection for the polynucleotide;
wherein the function is selected from one or more of:
(i) activation of macrophages,
(ii) activation of cytotoxic T-cells,
(iii) activation of B cells,
(iv) secretion of interferon gamma (IFN-γ), and
(v) secretion of tumor necrosis factor alpha (TNF-α).
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