	US 11,903,967 B2
	Method of preparing T cells with increased activity
Martin Pulé, London (GB); Carlotta Petticone, London (GB); James Faulkner, London (GB); Ekaterini Kotsopoulou, London (GB); Emma Chan, London (GB); and Richard Beswick, London (GB)
Assigned to AUTOLUS LIMITED, London (GB)
Filed by AUTOLUS LIMITED, London (GB)
Filed on Nov. 12, 2018, as Appl. No. 16/188,185.
Prior Publication US 2019/0209612 A1, Jul. 11, 2019
Int. Cl. A61K 35/17 (2015.01); C12N 15/85 (2006.01); A61K 38/17 (2006.01); A61P 35/02 (2006.01); C12N 5/0783 (2010.01); C12N 5/00 (2006.01); C07K 14/725 (2006.01)

CPC A61K 35/17 (2013.01) [A61K 38/177 (2013.01); A61P 35/02 (2018.01); C12N 5/0087 (2013.01); C12N 5/0636 (2013.01); C12N 5/0638 (2013.01); C12N 15/8509 (2013.01); C07K 14/7051 (2013.01); C12N 2015/8518 (2013.01); C12N 2501/599 (2013.01); C12N 2510/00 (2013.01); C12N 2810/6054 (2013.01); C12N 2810/6081 (2013.01)]

7 Claims

1. A method of preparing a population of genetically modified T cells which comprise a chimeric antigen receptor (CAR) or a transgenic T-cell receptor (TCR) comprising:

providing a starting population of T cells,

depleting said starting population of T cells which express a target antigen, and

introducing into the T cells in the depleted starting population a nucleic acid sequence which encodes a CAR or transgenic TCR against the target antigen,

wherein the target antigen is TCR beta constant region 1 (TRBC1) or TCR beta constant region 2 (TRBC2), and

wherein said genetically modified T cells are less exhausted compared with genetically modified T cells prepared without depleting said starting population of T cells which express the target antigen of the CAR or transgenic TCR.