US 12,229,674 B2
Method and illumination apparatus of the adaptive optics in reflection microscopy
Johannes Dominik Seelig, Bonn (DE); and Ivan Vishniakou, Bonn (DE)
Assigned to MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V., Munich (DE)
Appl. No. 17/917,254
Filed by MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V., Munich (DE)
PCT Filed Apr. 1, 2021, PCT No. PCT/EP2021/058599
§ 371(c)(1), (2) Date Oct. 6, 2022,
PCT Pub. No. WO2021/204663, PCT Pub. Date Oct. 14, 2021.
Claims priority of application No. 10 2020 109 734.4 (DE), filed on Apr. 7, 2020.
Prior Publication US 2023/0098493 A1, Mar. 30, 2023
Int. Cl. G06N 3/08 (2023.01); G01N 21/64 (2006.01); G02B 21/00 (2006.01); G02B 21/36 (2006.01)
CPC G06N 3/08 (2013.01) [G01N 21/6458 (2013.01); G02B 21/0072 (2013.01); G02B 21/0076 (2013.01); G02B 21/367 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for training a mathematical model which describes a light propagation in a reflection microscopy, the method comprising:
a step a) of radiating a light distribution I0 using an illumination unit which is assigned to a microscope into an excitation path of the microscope;
a step b) of modulating the light distribution I0 to form a light distribution IA in the excitation path via an optical modulator, wherein the optical modulator provides a light modulation MA;
a step c) of reflecting the light distribution IA at a location of a sample in a detection path of the microscope;
a step d) of modulating the light distribution IA to form a light distribution ID in the detection path via a further optical modulator, wherein the further optical modulator provides a light modulation MD;
a step e) of recording a reflected light distribution ID;
repeating steps a) to e) n-fold so as to generate an n-fold 3-tuple (MA, MD; ID);
transferring the n-fold 3-tuple (MA, MD; ID) to a computer so as to implement a mathematical model F for a light propagation in reflection microscopy; and
ascertaining the mathematical model F which describes the light propagation in reflection microscopy based on the n-fold 3-tuple (MA, MD; ID).