	US 12,228,575 B2
	Biomarker composition for predicting cancer malignant prognosis induced by microplastic exposure
Jin Su Kim, Seoul (KR); Hyeongi Kim, Seoul (KR); and Javeria Zaheer, Seoul (KR)
Assigned to KOREA INSTITUTE OF RADIOLOGICAL & MEDICAL SCIENCES, Seoul (KR)
Filed by KOREA INSTITUTE OF RADIOLOGICAL & MEDICAL SCIENCES, Seoul (KR)
Filed on Mar. 29, 2021, as Appl. No. 17/214,966.
Claims priority of application No. 10-2020-0038278 (KR), filed on Mar. 30, 2020.
Prior Publication US 2021/0301351 A1, Sep. 30, 2021
Int. Cl. C12Q 1/6886 (2018.01); G01N 33/574 (2006.01)

CPC G01N 33/57446 (2013.01) [C12Q 1/6886 (2013.01); G01N 33/574 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/158 (2013.01); G01N 2333/70532 (2013.01); G01N 2333/70585 (2013.01); G01N 2333/70596 (2013.01); G01N 2333/726 (2013.01); G01N 2333/91017 (2013.01)]

2 Claims

1. A method of detecting changes in protein expression in gastric cancer cells exposed to microplastic, comprising:

culturing gastric cancer cell lines obtained from subjects in medium;

treating the cultured gastric cancer cell lines with 500 ppm of the microplastic for 4 weeks; and

detecting protein levels of CD44, E-cadherin, N-cadherin, and PD-L1 (programmed death-ligand 1) in the cultured gastric cancer cell lines treated with the microplastic.