	US 12,228,570 B2
	Analyte quantitation
Dayong Jin, Ultimo (AU); and Hao He, Ultimo (AU)
Assigned to UNIVERSITY OF TECHNOLOGY SYDNEY, Ultimo (AU)
Appl. No. 17/279,576
Filed by UNIVERSITY OF TECHNOLOGY SYDNEY, Ultimo (AU)
PCT Filed Sep. 25, 2019, PCT No. PCT/AU2019/051034 § 371(c)(1), (2) Date Mar. 24, 2021, PCT Pub. No. WO2020/061632, PCT Pub. Date Apr. 2, 2020.
Claims priority of application No. 2018903614 (AU), filed on Sep. 25, 2018.
Prior Publication US 2021/0396746 A1, Dec. 23, 2021
Int. Cl. G01N 33/543 (2006.01); G01N 33/53 (2006.01)

CPC G01N 33/54388 (2021.08) [G01N 33/5302 (2013.01); G01N 33/54346 (2013.01); G01N 2333/705 (2013.01); G01N 2458/40 (2013.01)]

19 Claims

1. A method for determining the quantity of at least one analyte in a sample, the method comprising:

providing a lateral flow strip comprising a capture moiety and a conjugate comprising a detection moiety capable of binding the at least one analyte and highly-doped upconversion nanoparticles for visualising interaction of the at least one analyte and the capture moiety;

applying the sample to the lateral flow strip such that the conjugate binds the analyte to provide a bound analyte that is subsequently captured by the capture moiety; providing a testing device comprising an excitation light source configured to elicita detectable signal from the highly-doped upconversion nanoparticles and a detector to capture the detectable signal, the testing device being capable of receiving the lateral flow strip;

inserting the lateral flow strip to which the sample has been applied into the testing device;

irradiating an area of the lateral flow strip comprising the highly-doped upconversion nanoparticles with a beam of light so as to elicit a detectable signal from the highly-doped upconversion nanoparticles; and detecting the detectable signal and determining the quantity of the at least one analyte in the sample based on the detectable signal, wherein the highly-doped upconversion nanoparticles comprise a host material, a sensitiser which is Yb³⁺ or Er³⁺, and an activator which is Er³⁺, Tm³⁺, or Ho³⁺, and wherein the sensitiser is present in a concentration of at least 30 mol % and the activator is present in a concentration of at least 3 mol %, or

wherein the highly-doped upconversion nanoparticles comprise a host material and an activator which is Er³⁺ in a concentration of at least 50-8 mol %.